Clinicopathologic characteristics related to the high variability of coding mononucleotide repeat sequences in tumors with high-microsatellite instability

Kyung Young Chung, Nam Gyun Kim, Long Shan Li, Haeryoung Kim, Hyunki Kim, Chung Mo Nam, Hoguen Kim, Dong Hwan Shin

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Frameshift mutation at coding mononucleotide repeat sequences are common in tumors with microsatellite instability (MSI-H), but the incidences are different among the target genes. We analyzed the mutational profiles of 12 known target genes containing polydeoxyadenosine repeats in their coding sequences in 39 MSI-H colorectal carcinomas and 40 MSI-H gastric carcinomas by using polymerase chain reaction and sequencing, and compared the results with the clinicopathologic characteristics. Frameshift mutations of target genes in the MSI-H colorectal and gastric carcinomas are increased according to the length of the polydeoxyadenosine repeats in the target genes. The mean mutational rates of MSI-H colorectal carcinomas and MSI-H gastric carcinomas were 2.03 and 1.95 in the 4 genes containing (A) 10 repeats, 1.23 and 0.73 in the 4 genes with (A)9 repeats and 0.61 and 0.48 in the 4 genes containing (A)8 repeats, respectively (p<0.001). Among the evaluated clinicopathologic findings, intestinal type gastric carcinomas had more frameshift mutations than the diffuse type carcinomas (3.5 vs. 1.9, p=0.01). These findings suggest that mutational rates of the target genes in MSI-H tumors are diverse, and higher mutational rates are related to the length of mononucleotide repeat sequences of the target genes and histologic type of tumors.

Original languageEnglish (US)
Pages (from-to)439-444
Number of pages6
JournalOncology reports
Volume10
Issue number2
StatePublished - Mar 1 2003

Keywords

  • Frameshift mutation
  • MSI-H colorectal carcinoma
  • MSI-H gastric carcinoma
  • Polydeoxyadenosine repeat sequences

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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