Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis

Brian W. Biegler, Shirley X. Yan, Sterling B. Ortega, Deepani K. Tennakoon, Michael K. Racke, Nitin J. Karandikar

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Patients with multiple sclerosis (MS) show a high prevalence of myelin-reactive CD8+ and CD4+ T-cell responses, which are the putative effectors/modulators of CNS neuropathology. Utilizing a novel combination of short-term culture, CFSE-based sorting and anchored PCR, we evaluated clonal compositions of neuroantigen-targeting T-cells from RRMS patients and controls. CDR3 region analysis of TCRβ chains revealed biased use of specific TCRBV-bearing CD4+ clones. CD8+ clones showed homology to published TCR from CNS-infiltrating T-cells in MS lesions. These studies are the first description of TCR usage of CNS-specific CD8+ T-cells and provide insights into their potential regulatory role in disease.

Original languageEnglish (US)
Pages (from-to)131-140
Number of pages10
JournalJournal of Neuroimmunology
Volume234
Issue number1-2
DOIs
StatePublished - May 1 2011

Keywords

  • CD4
  • CD8
  • CDR3
  • Multiple sclerosis
  • Myelin
  • TCR

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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