Cloning and characterization of a novel hepatitis B virus x binding protein that inhibits viral replication

Margherita Melegari, Pier Paolo Scaglioni, Jack R. Wands

Research output: Contribution to journalArticle

187 Citations (Scopus)

Abstract

The hepatitis B virus and the mammalian hepadnavirus genomes encode for a short open reading frame called x. Expression of the protein product (HBx) appears necessary for establishment of natural infection. However, in vitro studies have suggested a multifunctional role for HBx as an indirect transcriptional transactivator of a variety of different viral and cellular promoters. Indeed, HBx has no known direct DNA binding properties but may interact with transcription factors as well as activate intracellular signaling pathways associated with cell growth. To further address the possible functional role of HBx in the life cycle of hepatitis B virus, we performed an analysis using the yeast two-hybrid system to screen a cDNA library derived from a hepatocellular carcinoma cell line with a HBx fusion bait in an attempt to identify cellular partners that may bind to and alter the biologic properties of HBx. A HBx-interacting protein that specifically complexes with the carboxy terminus of wild-type HBx was identified and designated XIP. This 9.6-kDa protein is capable of binding to HBx in vitro, and transient and stable expression in hepatocellular carcinoma cells abolishes the transactivation properties of HBx on luciferase constructs driven by AP-1 and endogenous hepatitis B virus enhancer/promoter elements. Investigation of the role of XIP in hepatitis B virus replication in differentiated hepatocellular carcinoma cells revealed that XIP expression reduces wild-type hepatitis B virus replication to levels observed following transfection with an HBx-minus virus. In contrast, the replication levels of the duck hepatitis B virus, a hepadnavirus that lacks the x open reading frame, were unchanged in the context of XIP expression. We propose that one of the physiologic functions of the cellular protein XIP is to negatively regulate HBx activity and thus to alter the replication life cycle of the virus.

Original languageEnglish (US)
Pages (from-to)1737-1743
Number of pages7
JournalJournal of Virology
Volume72
Issue number3
StatePublished - Mar 1998

Fingerprint

Virus Attachment
Hepatitis B virus
virus replication
binding proteins
Organism Cloning
molecular cloning
Carrier Proteins
Hepadnaviridae
hepatoma
Hepatocellular Carcinoma
Virus Replication
Life Cycle Stages
Open Reading Frames
open reading frames
Duck hepatitis B virus
life cycle (organisms)
Proteins
Duck Hepatitis B Viruses
promoter regions
Viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Cloning and characterization of a novel hepatitis B virus x binding protein that inhibits viral replication. / Melegari, Margherita; Scaglioni, Pier Paolo; Wands, Jack R.

In: Journal of Virology, Vol. 72, No. 3, 03.1998, p. 1737-1743.

Research output: Contribution to journalArticle

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