Cloning and characterization of human estrogen receptor β isoforms

John T. Moore, David D. McKee, Kimberly Slentz-Kesler, Linda B. Moore, Stacey A. Jones, Earnest L. Horne, Jui Lan Su, Steven A. Kliewer, Jürgen M. Lehmann, Timothy M. Willson

Research output: Contribution to journalArticle

439 Scopus citations

Abstract

Multiple transcripts which arise from the human estrogen receptor β (ERβ) gene have been characterized. Three full length isoforms of the hERβ gene, designated hERβ1-3, were identified in a testis cDNA library. An additional two isoforms, designated hERβ4 and hERβ5, were identified by PCR amplification from testis cDNA and from the MDA-MB 435 cell line. hERβ1 corresponds to the previously described hERβ. All five isoforms diverge at a common position within the predicted helix 10 of the ligand binding domain of hERβ, with nucleotide sequences consistent with differential exon usage. The hERβ isoform mRNAs displayed a differential pattern of expression in human tissues and in tumor cell lines when analyzed by RT-PCR. Further characterization of the three full length isoforms, hERβ1-3, by in vitro band shift studies indicated that the isoforms were able to form DNA-binding homodimers and heterodimers with each other and with the ERα subtype.

Original languageEnglish (US)
Pages (from-to)75-78
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume247
Issue number1
DOIs
StatePublished - Jun 9 1998

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Cloning and characterization of human estrogen receptor β isoforms'. Together they form a unique fingerprint.

  • Cite this

    Moore, J. T., McKee, D. D., Slentz-Kesler, K., Moore, L. B., Jones, S. A., Horne, E. L., Su, J. L., Kliewer, S. A., Lehmann, J. M., & Willson, T. M. (1998). Cloning and characterization of human estrogen receptor β isoforms. Biochemical and Biophysical Research Communications, 247(1), 75-78. https://doi.org/10.1006/bbrc.1998.8738