Cloning and characterization of the gene encoding the OmpU outer membrane protein of Vibrio cholerae

Vanessa Sperandio, Camella Bailey, Jorge A. Girón, Victor J. Dirita, Wanderley D. Silveira, André L. Vettore, James B. Kaper

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The OmpU outer membrane protein is a member of the ToxR regulon of Vibrio cholerae and has recently been shown to be a potential adherence factor for this species. Using PCR and degenerate oligonucleotide primers based on internal peptide sequences of purified OmpU, we have cloned and sequenced the gene encoding OmpU. The ompU gene is predicted to encode a 36,646-molecular-weight protein which is present in both cholera toxin- positive and -negative V. cholerae O1 and O139 strains.

Original languageEnglish (US)
Pages (from-to)5406-5409
Number of pages4
JournalInfection and Immunity
Volume64
Issue number12
StatePublished - 1996

Fingerprint

Vibrio cholerae
Organism Cloning
Membrane Proteins
Vibrio cholerae O139
Vibrio cholerae O1
Regulon
DNA Primers
Cholera Toxin
Genes
Molecular Weight
Polymerase Chain Reaction
Peptides
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Sperandio, V., Bailey, C., Girón, J. A., Dirita, V. J., Silveira, W. D., Vettore, A. L., & Kaper, J. B. (1996). Cloning and characterization of the gene encoding the OmpU outer membrane protein of Vibrio cholerae. Infection and Immunity, 64(12), 5406-5409.

Cloning and characterization of the gene encoding the OmpU outer membrane protein of Vibrio cholerae. / Sperandio, Vanessa; Bailey, Camella; Girón, Jorge A.; Dirita, Victor J.; Silveira, Wanderley D.; Vettore, André L.; Kaper, James B.

In: Infection and Immunity, Vol. 64, No. 12, 1996, p. 5406-5409.

Research output: Contribution to journalArticle

Sperandio, V, Bailey, C, Girón, JA, Dirita, VJ, Silveira, WD, Vettore, AL & Kaper, JB 1996, 'Cloning and characterization of the gene encoding the OmpU outer membrane protein of Vibrio cholerae', Infection and Immunity, vol. 64, no. 12, pp. 5406-5409.
Sperandio V, Bailey C, Girón JA, Dirita VJ, Silveira WD, Vettore AL et al. Cloning and characterization of the gene encoding the OmpU outer membrane protein of Vibrio cholerae. Infection and Immunity. 1996;64(12):5406-5409.
Sperandio, Vanessa ; Bailey, Camella ; Girón, Jorge A. ; Dirita, Victor J. ; Silveira, Wanderley D. ; Vettore, André L. ; Kaper, James B. / Cloning and characterization of the gene encoding the OmpU outer membrane protein of Vibrio cholerae. In: Infection and Immunity. 1996 ; Vol. 64, No. 12. pp. 5406-5409.
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