Cloning and characterization of the gene encoding the OmpU outer membrane protein of Vibrio cholerae

Vanessa Sperandio; Camella Bailey; Jorge A. Girón; Victor J. Dirita; Wanderley D. Silveira; André L. Vettore; James B. Kaper

doi:10.1128/iai.64.12.5406-5409.1996

Cloning and characterization of the gene encoding the OmpU outer membrane protein of Vibrio cholerae

Vanessa Sperandio, Camella Bailey, Jorge A. Girón, Victor J. Dirita, Wanderley D. Silveira, André L. Vettore, James B. Kaper

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

The OmpU outer membrane protein is a member of the ToxR regulon of Vibrio cholerae and has recently been shown to be a potential adherence factor for this species. Using PCR and degenerate oligonucleotide primers based on internal peptide sequences of purified OmpU, we have cloned and sequenced the gene encoding OmpU. The ompU gene is predicted to encode a 36,646-molecular-weight protein which is present in both cholera toxin- positive and -negative V. cholerae O1 and O139 strains.

Original language	English (US)
Pages (from-to)	5406-5409
Number of pages	4
Journal	Infection and immunity
Volume	64
Issue number	12
DOIs	https://doi.org/10.1128/iai.64.12.5406-5409.1996
State	Published - 1996

ASJC Scopus subject areas

Parasitology
Microbiology
Immunology
Infectious Diseases

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10.1128/iai.64.12.5406-5409.1996

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title = "Cloning and characterization of the gene encoding the OmpU outer membrane protein of Vibrio cholerae",

abstract = "The OmpU outer membrane protein is a member of the ToxR regulon of Vibrio cholerae and has recently been shown to be a potential adherence factor for this species. Using PCR and degenerate oligonucleotide primers based on internal peptide sequences of purified OmpU, we have cloned and sequenced the gene encoding OmpU. The ompU gene is predicted to encode a 36,646-molecular-weight protein which is present in both cholera toxin- positive and -negative V. cholerae O1 and O139 strains.",

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year = "1996",

doi = "10.1128/iai.64.12.5406-5409.1996",

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T1 - Cloning and characterization of the gene encoding the OmpU outer membrane protein of Vibrio cholerae

AU - Sperandio, Vanessa

AU - Bailey, Camella

AU - Girón, Jorge A.

AU - Dirita, Victor J.

AU - Silveira, Wanderley D.

AU - Vettore, André L.

AU - Kaper, James B.

PY - 1996

Y1 - 1996

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AB - The OmpU outer membrane protein is a member of the ToxR regulon of Vibrio cholerae and has recently been shown to be a potential adherence factor for this species. Using PCR and degenerate oligonucleotide primers based on internal peptide sequences of purified OmpU, we have cloned and sequenced the gene encoding OmpU. The ompU gene is predicted to encode a 36,646-molecular-weight protein which is present in both cholera toxin- positive and -negative V. cholerae O1 and O139 strains.

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JO - Infection and immunity

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Cloning and characterization of the gene encoding the OmpU outer membrane protein of Vibrio cholerae

Abstract

ASJC Scopus subject areas

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