Cloning and characterization of the gene encoding the OmpU outer membrane protein of Vibrio cholerae

Vanessa Sperandio, Camella Bailey, Jorge A. Girón, Victor J. Dirita, Wanderley D. Silveira, André L. Vettore, James B. Kaper

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Abstract

The OmpU outer membrane protein is a member of the ToxR regulon of Vibrio cholerae and has recently been shown to be a potential adherence factor for this species. Using PCR and degenerate oligonucleotide primers based on internal peptide sequences of purified OmpU, we have cloned and sequenced the gene encoding OmpU. The ompU gene is predicted to encode a 36,646-molecular-weight protein which is present in both cholera toxin- positive and -negative V. cholerae O1 and O139 strains.

Original languageEnglish (US)
Pages (from-to)5406-5409
Number of pages4
JournalInfection and Immunity
Volume64
Issue number12
Publication statusPublished - 1996

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ASJC Scopus subject areas

  • Immunology

Cite this

Sperandio, V., Bailey, C., Girón, J. A., Dirita, V. J., Silveira, W. D., Vettore, A. L., & Kaper, J. B. (1996). Cloning and characterization of the gene encoding the OmpU outer membrane protein of Vibrio cholerae. Infection and Immunity, 64(12), 5406-5409.