Cloning and chromosomal mapping of a human immunodeficiency virus 1 "TATA" element modulatory factor

Joseph A. Garcia, S. H Ignatius Ou, Foon Wu, Aldons J. Lusis, Robert S. Sparkes, Richard B. Gaynor

Research output: Contribution to journalArticle

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Abstract

A critical regulatory element in many promoters transcribed by RNA polymerase II is the "TATA" box, which is located 25-30 nucleotides upstream of the transcription initiation site. TFIID is a biochemically defined HeLa cell nuclear fraction containing a transcription factor activity that binds specifically to the TATA box and is critical in determining both basal and regulated promoter activity. Recently, the gene for a TATA-binding protein was cloned and found to bind to various TATA elements and to substitute for TFIID in stimulating basal gene expression in in vitro transcription systems. However, it is possible that additional cellular factors can bind to the TATA element and influence the level of gene expression. By using λgt11 expression cloning with oligonucleotides corresponding to the human immunodeficiency virus 1 TATA element, we report the identification of a cellular protein with a calculated molecular mass of 123 kDa that we designate TATA element modulatory factor (TMF). TMF binds to the human immunodeficiency virus 1 TATA element in gel-retardation assays and inhibits activation of the viral long terminal repeat by the TATA-binding protein in in vitro transcription assays. TMF contains leucine-zipper amino acid motifs and exhibits homology in its DNA binding domain with the phage-encoded DNA binding protein Ner. Chromosomal mapping localizes the TMF gene to human chromosome 3p12-p21, which is a site of frequent rearrangements in lung and renal carcinomas. Thus, TMF is a transcription factor that likely regulates the expression of both viral and cellular genes.

Original languageEnglish (US)
Pages (from-to)9372-9376
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number20
StatePublished - Oct 15 1992

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Transcription Factor TFIID
TATA-Box Binding Protein
TATA Box
HIV-1
Organism Cloning
Transcription Factors
Gene Expression
Virus Activation
Leucine Zippers
Amino Acid Motifs
Viral Genes
Terminal Repeat Sequences
RNA Polymerase II
Transcription Initiation Site
DNA-Binding Proteins
Human Chromosomes
Electrophoretic Mobility Shift Assay
HeLa Cells
Oligonucleotides
Bacteriophages

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Cloning and chromosomal mapping of a human immunodeficiency virus 1 "TATA" element modulatory factor. / Garcia, Joseph A.; Ou, S. H Ignatius; Wu, Foon; Lusis, Aldons J.; Sparkes, Robert S.; Gaynor, Richard B.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 20, 15.10.1992, p. 9372-9376.

Research output: Contribution to journalArticle

Garcia, Joseph A. ; Ou, S. H Ignatius ; Wu, Foon ; Lusis, Aldons J. ; Sparkes, Robert S. ; Gaynor, Richard B. / Cloning and chromosomal mapping of a human immunodeficiency virus 1 "TATA" element modulatory factor. In: Proceedings of the National Academy of Sciences of the United States of America. 1992 ; Vol. 89, No. 20. pp. 9372-9376.
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