Cloning and expression of a novel, truncated, progesterone receptor

Karla J. Saner, Brenda H. Welter, Fan Zhang, Elizabeth Hansen, Barbara Dupont, Yangzhan Wei, Thomas M. Price

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Progesterone acts via two specific receptors to affect gene transcription in target tissues. Progesterone receptor (PR) B contains 933 amino acids while PR A is a truncated version lacking the initial 164 amino acids. We have cloned a novel, truncated PR from both human adipose and aortic cDNA libraries. This cDNA encodes a predicted protein of 314 amino acids, termed PR-M. Initiation of transcription of PR-M occurs in intron 3, with the initial exon identical to exon 4 of the genomic PRs, except for a novel 16 amino acid amino-terminal sequence, consistent with a signal peptide. The remainder of PR-M is identical to the genomic PR. Transcript for this protein was identified by RT-PCR in human aortic endothelial cells and T47D breast cancer cells. Expression of PR-M in Sf 9 insect cells results in a 38-kDa protein, demonstrated in human aortic endothelial cells and T47D breast cancer cells. The function of PR-M remains to be determined. The presence of a signal peptide and the lack of a DNA binding region suggests a non-genomic action.

Original languageEnglish (US)
Pages (from-to)155-163
Number of pages9
JournalMolecular and Cellular Endocrinology
Volume200
Issue number1-2
DOIs
StatePublished - Feb 28 2003

Fingerprint

Cloning
Progesterone Receptors
Organism Cloning
Amino Acids
Endothelial cells
Transcription
Protein Sorting Signals
Exons
Endothelial Cells
Cells
Breast Neoplasms
Proteins
Gene Library
Introns
Progesterone
Insects
Complementary DNA
Genes
Tissue
Polymerase Chain Reaction

Keywords

  • Human aortic endothelial cells
  • Non-genomic
  • Progesterone receptor
  • T47D breast cancer cells
  • T47D-Y breast cancer cells

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Saner, K. J., Welter, B. H., Zhang, F., Hansen, E., Dupont, B., Wei, Y., & Price, T. M. (2003). Cloning and expression of a novel, truncated, progesterone receptor. Molecular and Cellular Endocrinology, 200(1-2), 155-163. https://doi.org/10.1016/S0303-7207(02)00380-5

Cloning and expression of a novel, truncated, progesterone receptor. / Saner, Karla J.; Welter, Brenda H.; Zhang, Fan; Hansen, Elizabeth; Dupont, Barbara; Wei, Yangzhan; Price, Thomas M.

In: Molecular and Cellular Endocrinology, Vol. 200, No. 1-2, 28.02.2003, p. 155-163.

Research output: Contribution to journalArticle

Saner, KJ, Welter, BH, Zhang, F, Hansen, E, Dupont, B, Wei, Y & Price, TM 2003, 'Cloning and expression of a novel, truncated, progesterone receptor', Molecular and Cellular Endocrinology, vol. 200, no. 1-2, pp. 155-163. https://doi.org/10.1016/S0303-7207(02)00380-5
Saner, Karla J. ; Welter, Brenda H. ; Zhang, Fan ; Hansen, Elizabeth ; Dupont, Barbara ; Wei, Yangzhan ; Price, Thomas M. / Cloning and expression of a novel, truncated, progesterone receptor. In: Molecular and Cellular Endocrinology. 2003 ; Vol. 200, No. 1-2. pp. 155-163.
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