Cloning and expression of a widely distributed (type IV) adenylyl cyclase

Boning Gao, Alfred G. Gilman

Research output: Contribution to journalArticle

268 Citations (Scopus)

Abstract

We have cloned and expressed a cDNA that encodes a widely distributed form of mammalian adenylyl cyclase (EC 4.6.1.1). Although those adenylyl cyclases described previously have a rather narrow tissue distribution, this enzyme (type IV) is apparently synthesized in a variety of peripheral tissues and in the central nervous system. The protein resembles the other adenylyl cyclases in its proposed structure. It most resembles the type II adenylyl cyclase described in the preceding paper [Feinstein, P. G., Schrader, K. A., Bakalyar, H. A., Tang, W.-J., Krupinski, J., Gilman, A. G. & Reed, R. R. (1991) Proc. Natl. Acad. Sci. USA 88, 10173-10177] in its amino acid sequence, lack of response to calmodulin, and synergistic activation by a combination of the Gs α subunit and the G-protein βγ subunit complex. (.

Original languageEnglish (US)
Pages (from-to)10178-10182
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number22
StatePublished - 1991

Fingerprint

Adenylyl Cyclases
Organism Cloning
Protein Subunits
Tissue Distribution
Calmodulin
GTP-Binding Proteins
Amino Acid Sequence
Central Nervous System
Complementary DNA
adenylyl cyclase 4
Enzymes
Proteins

Keywords

  • Cyclic AMP
  • Guanine nucleotide-binding protein
  • Signal transduction

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Cloning and expression of a widely distributed (type IV) adenylyl cyclase. / Gao, Boning; Gilman, Alfred G.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 22, 1991, p. 10178-10182.

Research output: Contribution to journalArticle

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