Cloning and expression of a widely distributed (type IV) adenylyl cyclase

Boning Gao, Alfred G. Gilman

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Abstract

We have cloned and expressed a cDNA that encodes a widely distributed form of mammalian adenylyl cyclase (EC 4.6.1.1). Although those adenylyl cyclases described previously have a rather narrow tissue distribution, this enzyme (type IV) is apparently synthesized in a variety of peripheral tissues and in the central nervous system. The protein resembles the other adenylyl cyclases in its proposed structure. It most resembles the type II adenylyl cyclase described in the preceding paper [Feinstein, P. G., Schrader, K. A., Bakalyar, H. A., Tang, W.-J., Krupinski, J., Gilman, A. G. and Reed, R. R. (1991) Proc. Natl. Acad. Sci. USA 88, 10173-10177] in its amino acid sequence, lack of response to calmodulin, and synergistic activation by a combination of the G(s) α subunit and the G-protein βγ subunit complex.

Original languageEnglish (US)
Pages (from-to)10178-10182
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number22
DOIs
StatePublished - Jan 1 1991

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Keywords

  • cyclic AMP
  • guanine nucleotide-binding protein
  • signal transduction

ASJC Scopus subject areas

  • General

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