Cloning and expression of full-length cDNA encoding human vitamin D receptor

A. R. Baker, D. P. McDonnell, M. Hughes, T. M. Crisp, D. J. Mangelsdorf, M. R. Haussler, J. W. Pike, J. Shine, B. W. O'Malley

Research output: Contribution to journalArticle

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Abstract

Complementary DNA clones encoding the human vitamin D receptor have been isolated from human intestine and T47D cell cDNA libraries. The nucleotide sequence of the 4605-base pair (bp) cDNA includes a noncoding leader sequence of 115 bp, a 1281-bp open reading frame, and 3209 bp of 3' noncoding sequence. Two polyadenylylation signals, AATAAA, are present 25 and 70 bp upstream of the poly(A) tail, respectively. RNA blot hybridization indicates a single mRNA species of ~4600 bp. Transfection of the cloned sequences into COS-1 cells results in the production of a single receptor species indistinguishable from the native receptor. Sequence comparison demonstrate that the vitamin D receptor belongs to the steroid-receptor gene family and is closest in size and sequence to another member of this family, the thyroid hormone receptor.

Original languageEnglish (US)
Pages (from-to)3294-3298
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number10
StatePublished - 1988

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Calcitriol Receptors
Base Pairing
Organism Cloning
Complementary DNA
Thyroid Hormone Receptors
Messenger RNA
Steroid Receptors
COS Cells
Gene Library
Open Reading Frames
Intestines
Transfection
Clone Cells
RNA
Genes

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Baker, A. R., McDonnell, D. P., Hughes, M., Crisp, T. M., Mangelsdorf, D. J., Haussler, M. R., ... O'Malley, B. W. (1988). Cloning and expression of full-length cDNA encoding human vitamin D receptor. Proceedings of the National Academy of Sciences of the United States of America, 85(10), 3294-3298.

Cloning and expression of full-length cDNA encoding human vitamin D receptor. / Baker, A. R.; McDonnell, D. P.; Hughes, M.; Crisp, T. M.; Mangelsdorf, D. J.; Haussler, M. R.; Pike, J. W.; Shine, J.; O'Malley, B. W.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 10, 1988, p. 3294-3298.

Research output: Contribution to journalArticle

Baker, AR, McDonnell, DP, Hughes, M, Crisp, TM, Mangelsdorf, DJ, Haussler, MR, Pike, JW, Shine, J & O'Malley, BW 1988, 'Cloning and expression of full-length cDNA encoding human vitamin D receptor', Proceedings of the National Academy of Sciences of the United States of America, vol. 85, no. 10, pp. 3294-3298.
Baker, A. R. ; McDonnell, D. P. ; Hughes, M. ; Crisp, T. M. ; Mangelsdorf, D. J. ; Haussler, M. R. ; Pike, J. W. ; Shine, J. ; O'Malley, B. W. / Cloning and expression of full-length cDNA encoding human vitamin D receptor. In: Proceedings of the National Academy of Sciences of the United States of America. 1988 ; Vol. 85, No. 10. pp. 3294-3298.
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AU - McDonnell, D. P.

AU - Hughes, M.

AU - Crisp, T. M.

AU - Mangelsdorf, D. J.

AU - Haussler, M. R.

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AU - Shine, J.

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