Cloning and expression of full-length cDNA encoding human vitamin D receptor

A. R. Baker; D. P. McDonnell; M. Hughes; T. M. Crisp; D. J. Mangelsdorf; M. R. Haussler; J. W. Pike; J. Shine; B. W. O'Malley

doi:10.1073/pnas.85.10.3294

Cloning and expression of full-length cDNA encoding human vitamin D receptor

A. R. Baker, D. P. McDonnell, M. Hughes, T. M. Crisp, D. J. Mangelsdorf, M. R. Haussler, J. W. Pike, J. Shine, B. W. O'Malley

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Abstract

Complementary DNA clones encoding the human vitamin D receptor have been isolated from human intestine and T47D cell cDNA libraries. The nucleotide sequence of the 4605-base pair (bp) cDNA includes a noncoding leader sequence of 115 bp, a 1281-bp open reading frame, and 3209 bp of 3' noncoding sequence. Two polyadenylylation signals, AATAAA, are present 25 and 70 bp upstream of the poly(A) tail, respectively. RNA blot hybridization indicates a single mRNA species of ~4600 bp. Transfection of the cloned sequences into COS-1 cells results in the production of a single receptor species indistinguishable from the native receptor. Sequence comparison demonstrate that the vitamin D receptor belongs to the steroid-receptor gene family and is closest in size and sequence to another member of this family, the thyroid hormone receptor.

Original language	English (US)
Pages (from-to)	3294-3298
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	85
Issue number	10
DOIs	https://doi.org/10.1073/pnas.85.10.3294
State	Published - 1988

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title = "Cloning and expression of full-length cDNA encoding human vitamin D receptor",

abstract = "Complementary DNA clones encoding the human vitamin D receptor have been isolated from human intestine and T47D cell cDNA libraries. The nucleotide sequence of the 4605-base pair (bp) cDNA includes a noncoding leader sequence of 115 bp, a 1281-bp open reading frame, and 3209 bp of 3' noncoding sequence. Two polyadenylylation signals, AATAAA, are present 25 and 70 bp upstream of the poly(A) tail, respectively. RNA blot hybridization indicates a single mRNA species of ~4600 bp. Transfection of the cloned sequences into COS-1 cells results in the production of a single receptor species indistinguishable from the native receptor. Sequence comparison demonstrate that the vitamin D receptor belongs to the steroid-receptor gene family and is closest in size and sequence to another member of this family, the thyroid hormone receptor.",

author = "Baker, {A. R.} and McDonnell, {D. P.} and M. Hughes and Crisp, {T. M.} and Mangelsdorf, {D. J.} and Haussler, {M. R.} and Pike, {J. W.} and J. Shine and O'Malley, {B. W.}",

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doi = "10.1073/pnas.85.10.3294",

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T1 - Cloning and expression of full-length cDNA encoding human vitamin D receptor

AU - Baker, A. R.

AU - McDonnell, D. P.

AU - Hughes, M.

AU - Crisp, T. M.

AU - Mangelsdorf, D. J.

AU - Haussler, M. R.

AU - Pike, J. W.

AU - Shine, J.

AU - O'Malley, B. W.

PY - 1988

Y1 - 1988

N2 - Complementary DNA clones encoding the human vitamin D receptor have been isolated from human intestine and T47D cell cDNA libraries. The nucleotide sequence of the 4605-base pair (bp) cDNA includes a noncoding leader sequence of 115 bp, a 1281-bp open reading frame, and 3209 bp of 3' noncoding sequence. Two polyadenylylation signals, AATAAA, are present 25 and 70 bp upstream of the poly(A) tail, respectively. RNA blot hybridization indicates a single mRNA species of ~4600 bp. Transfection of the cloned sequences into COS-1 cells results in the production of a single receptor species indistinguishable from the native receptor. Sequence comparison demonstrate that the vitamin D receptor belongs to the steroid-receptor gene family and is closest in size and sequence to another member of this family, the thyroid hormone receptor.

AB - Complementary DNA clones encoding the human vitamin D receptor have been isolated from human intestine and T47D cell cDNA libraries. The nucleotide sequence of the 4605-base pair (bp) cDNA includes a noncoding leader sequence of 115 bp, a 1281-bp open reading frame, and 3209 bp of 3' noncoding sequence. Two polyadenylylation signals, AATAAA, are present 25 and 70 bp upstream of the poly(A) tail, respectively. RNA blot hybridization indicates a single mRNA species of ~4600 bp. Transfection of the cloned sequences into COS-1 cells results in the production of a single receptor species indistinguishable from the native receptor. Sequence comparison demonstrate that the vitamin D receptor belongs to the steroid-receptor gene family and is closest in size and sequence to another member of this family, the thyroid hormone receptor.

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JF - Proceedings of the National Academy of Sciences of the United States of America

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Cloning and expression of full-length cDNA encoding human vitamin D receptor

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