Cloning and genomic organization of beclin 1, a candidate tumor suppressor gene on chromosome 17q21

Vincent M. Aita, Xiao Huan Liang, V. V V S Murty, David L. Pincus, Weiping Yu, Eftihia Cayanis, Sergei Kalachikov, T. Conrad Gilliam, Beth Levine

Research output: Contribution to journalArticle

556 Scopus citations

Abstract

The beclin 1 (BECN1) gene encodes a 60-kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2. Previous studies indicate that beclin 1 maps to a region approximately 150 kb centromeric to BRCA1 on chromosome 17q21 that is commonly deleted in breast, ovarian, and prostate cancer. The complete cDNA sequence of beclin 1 encodes a 2098-bp transcript, with a 120-bp 5' UTR, 1353-bp coding region, and 625-bp 3' UTR. Hybridization screening of a human genomic PAC library identified PAC 45208, which contains the complete beclin 1 gene. Determination of the exon-intron structure of beclin 1 reveals 12 exons, ranging from 61 to 794 bp, which extend over 12 kb of the human genome. FISH analysis of human breast carcinoma cell lines using PAC 45208 as probe identified allelic beclin 1 deletions in 9 of 22 cell lines. Sequencing of genomic DNA from 10 of these cell lines revealed no mutations in coding regions or splice junctions. Additionally, Northern blot analysis of 11 cell lines did not identify any abnormalities in beclin 1 transcripts. These results indicate that human breast carcinoma cell lines frequently contain allelic deletions of beclin 1, but not beclin 1 coding mutations.

Original languageEnglish (US)
Pages (from-to)59-65
Number of pages7
JournalGenomics
Volume59
Issue number1
DOIs
StatePublished - Jul 1 1999

ASJC Scopus subject areas

  • Genetics

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    Aita, V. M., Liang, X. H., Murty, V. V. V. S., Pincus, D. L., Yu, W., Cayanis, E., Kalachikov, S., Gilliam, T. C., & Levine, B. (1999). Cloning and genomic organization of beclin 1, a candidate tumor suppressor gene on chromosome 17q21. Genomics, 59(1), 59-65. https://doi.org/10.1006/geno.1999.5851