Cloning and identification of exon-intron organization of the rat ATP-citrate lyase gene

Young Ah Moon; Kyung Sup Kim; Sahng Wook Park; Yoon Soo Kim

doi:10.1016/0167-4781(96)00067-X

Cloning and identification of exon-intron organization of the rat ATP-citrate lyase gene

Young Ah Moon, Kyung Sup Kim, Sahng Wook Park, Yoon Soo Kim

Molecular Genetics

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The rat ATP-citrate lyase gene was cloned and the complete exon-intron organization of the gene has been identified. The ATP-citrate lyase gene, spanning about 55 kb, is divided into 29 exons that range in size from 30 to 986 base pairs. The sequences bordering the splice site junctions universally follow the GT/AG rule. Reverse transcription-polymerase chain reaction showed two forms of ACL mRNA; the one containing complete exons and the other lacking exon 14 were found in the brain, kidney, mammary gland, lung and liver. Also, the-restriction fragment length polymorphisms were observed at intron 10 and intron 11 regions.

Original language	English (US)
Pages (from-to)	280-284
Number of pages	5
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1307
Issue number	3
DOIs	https://doi.org/10.1016/0167-4781(96)00067-X
State	Published - Jul 17 1996

Keywords

ATP-citrate lyase gene
Cloning
Exon-intron organization

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/0167-4781(96)00067-X

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title = "Cloning and identification of exon-intron organization of the rat ATP-citrate lyase gene",

abstract = "The rat ATP-citrate lyase gene was cloned and the complete exon-intron organization of the gene has been identified. The ATP-citrate lyase gene, spanning about 55 kb, is divided into 29 exons that range in size from 30 to 986 base pairs. The sequences bordering the splice site junctions universally follow the GT/AG rule. Reverse transcription-polymerase chain reaction showed two forms of ACL mRNA; the one containing complete exons and the other lacking exon 14 were found in the brain, kidney, mammary gland, lung and liver. Also, the-restriction fragment length polymorphisms were observed at intron 10 and intron 11 regions.",

keywords = "ATP-citrate lyase gene, Cloning, Exon-intron organization",

author = "Moon, {Young Ah} and Kim, {Kyung Sup} and Park, {Sahng Wook} and Kim, {Yoon Soo}",

note = "Funding Information: This study was supported by the NonDirected Research Fund for Research Institutes (1993), Korea Research Foun- dation, and by Research Instructor Research Grant (1995), Yonsei University, College of Medicine.",

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doi = "10.1016/0167-4781(96)00067-X",

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T1 - Cloning and identification of exon-intron organization of the rat ATP-citrate lyase gene

AU - Moon, Young Ah

AU - Kim, Kyung Sup

AU - Park, Sahng Wook

AU - Kim, Yoon Soo

N1 - Funding Information: This study was supported by the NonDirected Research Fund for Research Institutes (1993), Korea Research Foun- dation, and by Research Instructor Research Grant (1995), Yonsei University, College of Medicine.

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Y1 - 1996/7/17

N2 - The rat ATP-citrate lyase gene was cloned and the complete exon-intron organization of the gene has been identified. The ATP-citrate lyase gene, spanning about 55 kb, is divided into 29 exons that range in size from 30 to 986 base pairs. The sequences bordering the splice site junctions universally follow the GT/AG rule. Reverse transcription-polymerase chain reaction showed two forms of ACL mRNA; the one containing complete exons and the other lacking exon 14 were found in the brain, kidney, mammary gland, lung and liver. Also, the-restriction fragment length polymorphisms were observed at intron 10 and intron 11 regions.

AB - The rat ATP-citrate lyase gene was cloned and the complete exon-intron organization of the gene has been identified. The ATP-citrate lyase gene, spanning about 55 kb, is divided into 29 exons that range in size from 30 to 986 base pairs. The sequences bordering the splice site junctions universally follow the GT/AG rule. Reverse transcription-polymerase chain reaction showed two forms of ACL mRNA; the one containing complete exons and the other lacking exon 14 were found in the brain, kidney, mammary gland, lung and liver. Also, the-restriction fragment length polymorphisms were observed at intron 10 and intron 11 regions.

KW - ATP-citrate lyase gene

KW - Cloning

KW - Exon-intron organization

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JO - Biochimica et Biophysica Acta - Gene Structure and Expression

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Cloning and identification of exon-intron organization of the rat ATP-citrate lyase gene

Abstract

Keywords

ASJC Scopus subject areas

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