TY - JOUR
T1 - Cloning and initial characterization of mouse meltrin β and analysis of the expression of four metalloproteasedisintegrins in bone cells
AU - Inoue, Daisuke
AU - Reid, Martha
AU - Lum, Lawrence
AU - Krätzschmar, Jörn
AU - Weskamp, Gisela
AU - Myung, Yoon Mo
AU - Baron, Roland
AU - Blobel, Carl P.
PY - 1998/2/13
Y1 - 1998/2/13
N2 - Here we report the cloning and initial biochemical characterization of the mouse metalloprotease/disintegrin/cysteine-rich (MDC) protein meltrin β and the analysis of the mRNA expression of four MDC genes (meltrin α, meltrin β, mdc9, and mdc15) in bone cells, including osteoclasts and osteoblasts. Like most other MDC proteins, the predicted meltrin β protein consists of a signal sequence, prodomain, metalloprotease domain with a predicted catalytic site, disintegrin domain, cysteine-rich region, epidermal growth factor repeat, transmembrane domain, and cytoplasmic domain with putative signaling motifs, such as potential SH3 ligand domains. Northern blot analysis indicates that meltrin β is widely expressed, with the highest expression in bone, heart, and lung. RNase protection studies revealed expression of all four MDC genes analyzed here in osteoblasts, whereas only mdc9 and mdc15 mRNAs were detectable in osteoclast-like cells generated in vitro. Treatment of primary osteoblasts with 10 nM calcitriol increased meltrin β expression more than 3-fold, and both meltrin α and meltrin β expression is apparently regulated in a differentiation-associated manner in a mouse osteoblastic cell line, MC3T3E1. Collectively, these results suggest that meltrin α and meltrin β may play a role in osteoblast differentiation and/or function but are not likely to be involved in osteoclast fusion.
AB - Here we report the cloning and initial biochemical characterization of the mouse metalloprotease/disintegrin/cysteine-rich (MDC) protein meltrin β and the analysis of the mRNA expression of four MDC genes (meltrin α, meltrin β, mdc9, and mdc15) in bone cells, including osteoclasts and osteoblasts. Like most other MDC proteins, the predicted meltrin β protein consists of a signal sequence, prodomain, metalloprotease domain with a predicted catalytic site, disintegrin domain, cysteine-rich region, epidermal growth factor repeat, transmembrane domain, and cytoplasmic domain with putative signaling motifs, such as potential SH3 ligand domains. Northern blot analysis indicates that meltrin β is widely expressed, with the highest expression in bone, heart, and lung. RNase protection studies revealed expression of all four MDC genes analyzed here in osteoblasts, whereas only mdc9 and mdc15 mRNAs were detectable in osteoclast-like cells generated in vitro. Treatment of primary osteoblasts with 10 nM calcitriol increased meltrin β expression more than 3-fold, and both meltrin α and meltrin β expression is apparently regulated in a differentiation-associated manner in a mouse osteoblastic cell line, MC3T3E1. Collectively, these results suggest that meltrin α and meltrin β may play a role in osteoblast differentiation and/or function but are not likely to be involved in osteoclast fusion.
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U2 - 10.1074/jbc.273.7.4180
DO - 10.1074/jbc.273.7.4180
M3 - Article
C2 - 9461614
AN - SCOPUS:0032512831
SN - 0021-9258
VL - 273
SP - 4180
EP - 4187
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 7
ER -