Cloning and nucleotide sequence analysis of the human β-microseminoprotein gene

Carla B. Green; Whi Yu Liu; Simon C M Kwok

doi:10.1016/0006-291X(90)90648-7

Cloning and nucleotide sequence analysis of the human β-microseminoprotein gene

Carla B. Green, Whi Yu Liu, Simon C M Kwok

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Abstract

β-microseminoprotein (MSP) is a small protein (94 amino acids) synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. Restriction endonuclease mapping of human genomic DNA with a human MSP cDNA probe identified a 19 kilobase (Kb) hybridizing band in both EcoRI and BamHI digestions. Subsequently, the 19 Kb EcoRI fragment of human genomic DNA containing the MSP gene was isolated and cloned into an EMBL4 phage vector. Screening of the recombinant phages resulted in the isolation of one clone containing the MSP gene. Restriction endonuclease mapping and sequence analysis of this clone revealed the human MSP gene of approximately 15 Kb in length. The gene contains four exons and three large introns of approximately 6, 1, and 7 Kb.

Original language	English (US)
Pages (from-to)	1184-1190
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	167
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(90)90648-7
State	Published - Mar 30 1990

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Cloning and nucleotide sequence analysis of the human β-microseminoprotein gene",

abstract = "β-microseminoprotein (MSP) is a small protein (94 amino acids) synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. Restriction endonuclease mapping of human genomic DNA with a human MSP cDNA probe identified a 19 kilobase (Kb) hybridizing band in both EcoRI and BamHI digestions. Subsequently, the 19 Kb EcoRI fragment of human genomic DNA containing the MSP gene was isolated and cloned into an EMBL4 phage vector. Screening of the recombinant phages resulted in the isolation of one clone containing the MSP gene. Restriction endonuclease mapping and sequence analysis of this clone revealed the human MSP gene of approximately 15 Kb in length. The gene contains four exons and three large introns of approximately 6, 1, and 7 Kb.",

author = "Green, {Carla B.} and Liu, {Whi Yu} and Kwok, {Simon C M}",

note = "Funding Information: Acknowledgments: We gratefully acknowledge Dr. Mark Noble for providing us a surgical specimen of hyperplastic human prostate, and Dr. Donald Steiner for an oligonucleotide (30-mer) used in the initial isolation of the MSP cDNA. We also thank Mr. Kevin Graham for his assistance in the synthesis of oligonucleotides. The project was supported in part by BRSG SO7 RR05373, awarded by the Biomedical Research Support Grant Program, Division of Research Resources, National Institutes of Health. Other support includes grants from the American Cancer Society (#IN-l 15) through the University of Kansas Cancer Center, and from the Speas Foundation (#843548.15).",

year = "1990",

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doi = "10.1016/0006-291X(90)90648-7",

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T1 - Cloning and nucleotide sequence analysis of the human β-microseminoprotein gene

AU - Green, Carla B.

AU - Liu, Whi Yu

AU - Kwok, Simon C M

N1 - Funding Information: Acknowledgments: We gratefully acknowledge Dr. Mark Noble for providing us a surgical specimen of hyperplastic human prostate, and Dr. Donald Steiner for an oligonucleotide (30-mer) used in the initial isolation of the MSP cDNA. We also thank Mr. Kevin Graham for his assistance in the synthesis of oligonucleotides. The project was supported in part by BRSG SO7 RR05373, awarded by the Biomedical Research Support Grant Program, Division of Research Resources, National Institutes of Health. Other support includes grants from the American Cancer Society (#IN-l 15) through the University of Kansas Cancer Center, and from the Speas Foundation (#843548.15).

PY - 1990/3/30

Y1 - 1990/3/30

N2 - β-microseminoprotein (MSP) is a small protein (94 amino acids) synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. Restriction endonuclease mapping of human genomic DNA with a human MSP cDNA probe identified a 19 kilobase (Kb) hybridizing band in both EcoRI and BamHI digestions. Subsequently, the 19 Kb EcoRI fragment of human genomic DNA containing the MSP gene was isolated and cloned into an EMBL4 phage vector. Screening of the recombinant phages resulted in the isolation of one clone containing the MSP gene. Restriction endonuclease mapping and sequence analysis of this clone revealed the human MSP gene of approximately 15 Kb in length. The gene contains four exons and three large introns of approximately 6, 1, and 7 Kb.

AB - β-microseminoprotein (MSP) is a small protein (94 amino acids) synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. Restriction endonuclease mapping of human genomic DNA with a human MSP cDNA probe identified a 19 kilobase (Kb) hybridizing band in both EcoRI and BamHI digestions. Subsequently, the 19 Kb EcoRI fragment of human genomic DNA containing the MSP gene was isolated and cloned into an EMBL4 phage vector. Screening of the recombinant phages resulted in the isolation of one clone containing the MSP gene. Restriction endonuclease mapping and sequence analysis of this clone revealed the human MSP gene of approximately 15 Kb in length. The gene contains four exons and three large introns of approximately 6, 1, and 7 Kb.

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JO - Biochemical and Biophysical Research Communications

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Cloning and nucleotide sequence analysis of the human β-microseminoprotein gene

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