Cloning and overexpression of a tyrosinase gene mel from Pseudomonas maltophila

Gelin Wang, Aberrahmane Aazaz, Zhenrong Peng, Ping Shen

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

The tyrosinase gene (mel), which is responsible for melanin formation, was isolated by shotgun cloning of SalI fragments of Pseudomonas maltophila DNA. A 0.7-kb SalI fragment in the recombinant plasmid pWSY8 imparted the ability to synthesize melanin to an Escherichia coli host HB101. The nucleotide sequence of this DNA fragment revealed an open reading frame of 504 bp, encoding a protein of 169 amino acids. The fragment containing the mel gene was then cloned into an expression plasmid pPAS1 under the control of a promoter isolated from the host, P. maltophilia AT18. This strain increased the melanin production by 70.6% compared with the strain HB101/pWSY8, in which the cloned mel gene was under the control of the lac promoter from the vector pUC18. Copyright (C) 2000 Federation of European Microbiological Societies.

Original languageEnglish (US)
Pages (from-to)23-27
Number of pages5
JournalFEMS Microbiology Letters
Volume185
Issue number1
DOIs
StatePublished - Apr 1 2000

Keywords

  • Melanin
  • Promoter
  • Pseudomonas maltophila
  • mel gene

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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