Cloning and sequence of the human α7 nicotinic acetylcholine receptor

L. Doucette-Stamm, Lisa M Monteggia, D. Donnelly-Roberts, M. T. Wang, J. Lee, J. Tian, T. Giordano

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A rat clone for the nicotinic acetylcholine receptor (nAChR) α7, generated by reverse transcriptase-polymerase chain reaction (rtPCR) from a region which did not exhibit significant homology to other rat nAChRs, was used to probe human cDNA libraries. A number of clones corresponding to the human α7 were isolated and sequenced. The human sequence showed 87% identity at the nucleic acid level to the rat sequence and 96% identity at the amino acid level. Unlike the rat α7 nAChR, the human gene does not appear to be the most divergent member of the nAChR family. The cloning of the human α7 will make it possible to study the functional formation of human neuronal nAChR channels containing the α7 subtype.

Original languageEnglish (US)
Pages (from-to)252-256
Number of pages5
JournalDrug Development Research
Volume30
Issue number4
StatePublished - 1993

Fingerprint

Cloning
Nicotinic Receptors
Rats
Organism Cloning
Clone Cells
Polymerase chain reaction
RNA-Directed DNA Polymerase
Gene Library
Nucleic Acids
Reverse Transcriptase Polymerase Chain Reaction
Genes
Amino Acids

Keywords

  • cloning
  • human
  • nicotinic acetylcholine receptor
  • rtPCR

ASJC Scopus subject areas

  • Organic Chemistry
  • Drug Discovery
  • Pharmacology

Cite this

Doucette-Stamm, L., Monteggia, L. M., Donnelly-Roberts, D., Wang, M. T., Lee, J., Tian, J., & Giordano, T. (1993). Cloning and sequence of the human α7 nicotinic acetylcholine receptor. Drug Development Research, 30(4), 252-256.

Cloning and sequence of the human α7 nicotinic acetylcholine receptor. / Doucette-Stamm, L.; Monteggia, Lisa M; Donnelly-Roberts, D.; Wang, M. T.; Lee, J.; Tian, J.; Giordano, T.

In: Drug Development Research, Vol. 30, No. 4, 1993, p. 252-256.

Research output: Contribution to journalArticle

Doucette-Stamm, L, Monteggia, LM, Donnelly-Roberts, D, Wang, MT, Lee, J, Tian, J & Giordano, T 1993, 'Cloning and sequence of the human α7 nicotinic acetylcholine receptor', Drug Development Research, vol. 30, no. 4, pp. 252-256.
Doucette-Stamm L, Monteggia LM, Donnelly-Roberts D, Wang MT, Lee J, Tian J et al. Cloning and sequence of the human α7 nicotinic acetylcholine receptor. Drug Development Research. 1993;30(4):252-256.
Doucette-Stamm, L. ; Monteggia, Lisa M ; Donnelly-Roberts, D. ; Wang, M. T. ; Lee, J. ; Tian, J. ; Giordano, T. / Cloning and sequence of the human α7 nicotinic acetylcholine receptor. In: Drug Development Research. 1993 ; Vol. 30, No. 4. pp. 252-256.
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