Cloning and sequencing of the gene encoding the Cu,Zn-superoxide dismutase of Haemophilus ducreyi

Marla K. Stevens; Daniel J. Hassett; Justin D. Radolf; Eric J. Hansen

doi:10.1016/S0378-1119(96)00417-9

Cloning and sequencing of the gene encoding the Cu,Zn-superoxide dismutase of Haemophilus ducreyi

Marla K. Stevens, Daniel J. Hassett, Justin D. Radolf, Eric J. Hansen

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The sodC gene of Haemophilus ducreyi was cloned and sequenced. The deduced amino acid sequence of this protein exhibited 71.6% identity and 81.8% similarity to the H. influenzae and H. parainfluenzae copper (Cu), zinc (Zn)-superoxide dismutase (SOD) enzymes. This gene was localized to a 2.2-kb H. ducreyi chromosomal DNA insert in plasmid pHdSOD. SOD activity was expressed in cell-free extracts of Escherichia coli containing the recombinant plasmid pHdSOD and was localized to the periplasmic space. The Cu,Zn-SOD produced by the H. ducreyi sodC gene did not complement the aerobic growth defect of an E. coli SOD-deficient mutant.

Original language	English (US)
Pages (from-to)	35-40
Number of pages	6
Journal	Gene
Volume	183
Issue number	1-2
DOIs	https://doi.org/10.1016/S0378-1119(96)00417-9
State	Published - 1996

Keywords

Mmetalloenzyme
sodC

ASJC Scopus subject areas

Genetics

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10.1016/S0378-1119(96)00417-9

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title = "Cloning and sequencing of the gene encoding the Cu,Zn-superoxide dismutase of Haemophilus ducreyi",

abstract = "The sodC gene of Haemophilus ducreyi was cloned and sequenced. The deduced amino acid sequence of this protein exhibited 71.6% identity and 81.8% similarity to the H. influenzae and H. parainfluenzae copper (Cu), zinc (Zn)-superoxide dismutase (SOD) enzymes. This gene was localized to a 2.2-kb H. ducreyi chromosomal DNA insert in plasmid pHdSOD. SOD activity was expressed in cell-free extracts of Escherichia coli containing the recombinant plasmid pHdSOD and was localized to the periplasmic space. The Cu,Zn-SOD produced by the H. ducreyi sodC gene did not complement the aerobic growth defect of an E. coli SOD-deficient mutant.",

keywords = "Mmetalloenzyme, sodC",

author = "Stevens, {Marla K.} and Hassett, {Daniel J.} and Radolf, {Justin D.} and Hansen, {Eric J.}",

note = "Funding Information: This study was supported by Public Health Service grant AI 32011 to E.J.H. and J.D.R.",

year = "1996",

doi = "10.1016/S0378-1119(96)00417-9",

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volume = "183",

pages = "35--40",

journal = "Gene",

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publisher = "Elsevier",

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T1 - Cloning and sequencing of the gene encoding the Cu,Zn-superoxide dismutase of Haemophilus ducreyi

AU - Stevens, Marla K.

AU - Hassett, Daniel J.

AU - Radolf, Justin D.

AU - Hansen, Eric J.

N1 - Funding Information: This study was supported by Public Health Service grant AI 32011 to E.J.H. and J.D.R.

PY - 1996

Y1 - 1996

N2 - The sodC gene of Haemophilus ducreyi was cloned and sequenced. The deduced amino acid sequence of this protein exhibited 71.6% identity and 81.8% similarity to the H. influenzae and H. parainfluenzae copper (Cu), zinc (Zn)-superoxide dismutase (SOD) enzymes. This gene was localized to a 2.2-kb H. ducreyi chromosomal DNA insert in plasmid pHdSOD. SOD activity was expressed in cell-free extracts of Escherichia coli containing the recombinant plasmid pHdSOD and was localized to the periplasmic space. The Cu,Zn-SOD produced by the H. ducreyi sodC gene did not complement the aerobic growth defect of an E. coli SOD-deficient mutant.

AB - The sodC gene of Haemophilus ducreyi was cloned and sequenced. The deduced amino acid sequence of this protein exhibited 71.6% identity and 81.8% similarity to the H. influenzae and H. parainfluenzae copper (Cu), zinc (Zn)-superoxide dismutase (SOD) enzymes. This gene was localized to a 2.2-kb H. ducreyi chromosomal DNA insert in plasmid pHdSOD. SOD activity was expressed in cell-free extracts of Escherichia coli containing the recombinant plasmid pHdSOD and was localized to the periplasmic space. The Cu,Zn-SOD produced by the H. ducreyi sodC gene did not complement the aerobic growth defect of an E. coli SOD-deficient mutant.

KW - Mmetalloenzyme

KW - sodC

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DO - 10.1016/S0378-1119(96)00417-9

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AN - SCOPUS:0030443940

SN - 0378-1119

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Cloning and sequencing of the gene encoding the Cu,Zn-superoxide dismutase of Haemophilus ducreyi

Abstract

Keywords

ASJC Scopus subject areas

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