Cloning and sequencing of the gene encoding the Cu,Zn-superoxide dismutase of Haemophilus ducreyi

Marla K. Stevens, Daniel J. Hassett, Justin D. Radolf, Eric J. Hansen

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The sodC gene of Haemophilus ducreyi was cloned and sequenced. The deduced amino acid sequence of this protein exhibited 71.6% identity and 81.8% similarity to the H. influenzae and H. parainfluenzae copper (Cu), zinc (Zn)-superoxide dismutase (SOD) enzymes. This gene was localized to a 2.2-kb H. ducreyi chromosomal DNA insert in plasmid pHdSOD. SOD activity was expressed in cell-free extracts of Escherichia coli containing the recombinant plasmid pHdSOD and was localized to the periplasmic space. The Cu,Zn-SOD produced by the H. ducreyi sodC gene did not complement the aerobic growth defect of an E. coli SOD-deficient mutant.

Original languageEnglish (US)
Pages (from-to)35-40
Number of pages6
JournalGene
Volume183
Issue number1-2
DOIs
StatePublished - Dec 1 1996

Keywords

  • Mmetalloenzyme
  • sodC

ASJC Scopus subject areas

  • Genetics

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