Cloning and transient expression of genes encoding the human α4 and β2 neuronal nicotinic acetylcholine receptor (nAChR) subunits

Lisa M Monteggia; Murali Gopalakrishnan; Edward Touma; Kenneth B. Idler; Norman Nash; Stephen P. Arneric; James P. Sullivan; Tony Giordano

doi:10.1016/0378-1119(94)00914-E

Cloning and transient expression of genes encoding the human α4 and β2 neuronal nicotinic acetylcholine receptor (nAChR) subunits

Lisa M Monteggia, Murali Gopalakrishnan, Edward Touma, Kenneth B. Idler, Norman Nash, Stephen P. Arneric, James P. Sullivan, Tony Giordano

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Partial cDNA clones generated by RT-PCR were used as probes to clone the cDNAs encoding the human α4 and β2 neuronal nicotinic acetylcholine receptor (nAChR) subunits. The 2.1-kb α4 cDNA shows 84 and 76% identity to the rat and chicken cDNA sequences, respectively. The deduced amino-acid sequence shares 89 and 84% similarity, respectively, with the corresponding rat and chicken proteins, with most of the divergence occurring in the cytoplasmic domain. The 1721-nucleotide β sequence was identical to the human β2 sequence previously reported. Transfection of the α4 and β2 clones into HEK293 cells resulted in the formation of binding sites that display high affinity towards [³h]cytisine, a characteristic of the α4β2 subtype produced in vivo.

Original language	English (US)
Pages (from-to)	189-193
Number of pages	5
Journal	Gene
Volume	155
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(94)00914-E
State	Published - Apr 3 1995

Keywords

Ion channel
cDNA
cytisine
polymerase chain reaction
sequence homology

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(94)00914-E

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title = "Cloning and transient expression of genes encoding the human α4 and β2 neuronal nicotinic acetylcholine receptor (nAChR) subunits",

abstract = "Partial cDNA clones generated by RT-PCR were used as probes to clone the cDNAs encoding the human α4 and β2 neuronal nicotinic acetylcholine receptor (nAChR) subunits. The 2.1-kb α4 cDNA shows 84 and 76% identity to the rat and chicken cDNA sequences, respectively. The deduced amino-acid sequence shares 89 and 84% similarity, respectively, with the corresponding rat and chicken proteins, with most of the divergence occurring in the cytoplasmic domain. The 1721-nucleotide β sequence was identical to the human β2 sequence previously reported. Transfection of the α4 and β2 clones into HEK293 cells resulted in the formation of binding sites that display high affinity towards [3h]cytisine, a characteristic of the α4β2 subtype produced in vivo.",

keywords = "Ion channel, cDNA, cytisine, polymerase chain reaction, sequence homology",

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T1 - Cloning and transient expression of genes encoding the human α4 and β2 neuronal nicotinic acetylcholine receptor (nAChR) subunits

AU - Monteggia, Lisa M

AU - Gopalakrishnan, Murali

AU - Touma, Edward

AU - Idler, Kenneth B.

AU - Nash, Norman

AU - Arneric, Stephen P.

AU - Sullivan, James P.

AU - Giordano, Tony

PY - 1995/4/3

Y1 - 1995/4/3

N2 - Partial cDNA clones generated by RT-PCR were used as probes to clone the cDNAs encoding the human α4 and β2 neuronal nicotinic acetylcholine receptor (nAChR) subunits. The 2.1-kb α4 cDNA shows 84 and 76% identity to the rat and chicken cDNA sequences, respectively. The deduced amino-acid sequence shares 89 and 84% similarity, respectively, with the corresponding rat and chicken proteins, with most of the divergence occurring in the cytoplasmic domain. The 1721-nucleotide β sequence was identical to the human β2 sequence previously reported. Transfection of the α4 and β2 clones into HEK293 cells resulted in the formation of binding sites that display high affinity towards [3h]cytisine, a characteristic of the α4β2 subtype produced in vivo.

AB - Partial cDNA clones generated by RT-PCR were used as probes to clone the cDNAs encoding the human α4 and β2 neuronal nicotinic acetylcholine receptor (nAChR) subunits. The 2.1-kb α4 cDNA shows 84 and 76% identity to the rat and chicken cDNA sequences, respectively. The deduced amino-acid sequence shares 89 and 84% similarity, respectively, with the corresponding rat and chicken proteins, with most of the divergence occurring in the cytoplasmic domain. The 1721-nucleotide β sequence was identical to the human β2 sequence previously reported. Transfection of the α4 and β2 clones into HEK293 cells resulted in the formation of binding sites that display high affinity towards [3h]cytisine, a characteristic of the α4β2 subtype produced in vivo.

KW - Ion channel

KW - cDNA

KW - cytisine

KW - polymerase chain reaction

KW - sequence homology

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JF - Gene

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Cloning and transient expression of genes encoding the human α4 and β2 neuronal nicotinic acetylcholine receptor (nAChR) subunits

Abstract

Keywords

ASJC Scopus subject areas

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