Cloning of a Novel cDNA Homologous to CHIP28 Water Channel from Ocular Ciliary Epithelium

R. V. Patil, X. Yang, I. Saito, M. Cocaprados, M. B. Wax

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Water channel protein (CHIP28) has been initially identified from human erythrocyte and subsequently localized in human kidney, rat kidney and bovine corneal endothelium. We report here cloning of homologous cDNA (CHIP29) from an expression cDNA library constructed from bovine ciliary epithelium poly A+ that codes a 271 amino acid, 28.8 kDa protein. At the amino acid level, bovine CHIP29 is 91% identical to the CHIP28 protein. Analysis of the deduced amino acid sequence indicated a hydrophobic protein with six membrane spanning domains, one N-linked glycosylation site, two consented NPA boxes common to MIP26 family proteins, and conserved amino acid residue cysteine 189 common to water channels. Northern blotting revealed a major mRNA of 2.8 Kb size expressed in bovine ciliary epithelium. The results presented here confirm the presence of water channel gene in the bovine ciliary epithelium, a primary site for the aqueous humor production.

Original languageEnglish (US)
Pages (from-to)861-866
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume204
Issue number2
DOIs
StatePublished - Oct 31 1994

Fingerprint

Aquaporins
Cloning
Organism Cloning
Epithelium
Complementary DNA
Amino Acids
Aquaporin 1
Glycosylation
Proteins
Poly A
Kidney
Corneal Endothelium
Gene Library
Aqueous Humor
Cysteine
Protein Sequence Analysis
Rats
Genes
Northern Blotting
Membranes

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Cloning of a Novel cDNA Homologous to CHIP28 Water Channel from Ocular Ciliary Epithelium. / Patil, R. V.; Yang, X.; Saito, I.; Cocaprados, M.; Wax, M. B.

In: Biochemical and Biophysical Research Communications, Vol. 204, No. 2, 31.10.1994, p. 861-866.

Research output: Contribution to journalArticle

Patil, R. V. ; Yang, X. ; Saito, I. ; Cocaprados, M. ; Wax, M. B. / Cloning of a Novel cDNA Homologous to CHIP28 Water Channel from Ocular Ciliary Epithelium. In: Biochemical and Biophysical Research Communications. 1994 ; Vol. 204, No. 2. pp. 861-866.
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