Cloning of a second dendritic cell-associated C-type lectin (dectin-2) and its alternatively spliced isoforms

Kiyoshi Ariizumi, Guo Liang Shen, Sojin Shikano, Robert Ritter, Paul Zukas, Dale Edelbaum, Akimichi Morita, Akira Takashima

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

Using a subtractive cDNA cloning strategy, we isolated previously five novel genes that were expressed abundantly by the murine dendritic cell (DC) line XS52, but not by the J774 macrophage line. One of these genes encoded a unique, DC-associated C-type lectin, termed 'dectin-1.' Here we report the characterization of a second novel gene that was also expressed in a DC- specific manner. Clone 1B12 encoded a type H membrane-integrated polypeptide of 209 amino acids containing a single carbohydrate recognition domain motif in the COOH terminus. The expression pattern of this molecule, termed 'dectin-2,' was almost indistinguishable from that for dectin-1; that is, both were expressed abundantly at mRNA and protein levels by the XS52 DC line, but not by non-DC lines, and both were detected in spleen and thymus, as well as in skin resident DC (i.e. Langerhans cells). Interestingly, reverse transcriptase-polymerase chain reaction and immunoblotting revealed multiple bands of dectin-2 transcripts and proteins suggesting molecular heterogeneity. In fact, we isolated additional cDNA clones encoding two distinct, truncated dectin-2 isoforms. Genomic analyses indicated that a full-length dectin-2 (α isoform) is encoded by 6 exons, whereas truncated isoforms (β and γ) are produced by alternative splicing. We propose that dectin-2 and its isoforms, together with dectin-1, represent a unique subfamily of DC-associated C-type lectins.

Original languageEnglish (US)
Pages (from-to)11957-11963
Number of pages7
JournalJournal of Biological Chemistry
Volume275
Issue number16
DOIs
StatePublished - Apr 21 2000

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C-Type Lectins
Cloning
Dendritic Cells
Organism Cloning
Protein Isoforms
Langerhans Cells
Cell Line
Genes
Complementary DNA
Clone Cells
Alternative Splicing
Thymus
Reverse Transcriptase Polymerase Chain Reaction
Immunoblotting
Macrophages
Polymerase chain reaction
RNA-Directed DNA Polymerase
Thymus Gland
Exons
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Cloning of a second dendritic cell-associated C-type lectin (dectin-2) and its alternatively spliced isoforms. / Ariizumi, Kiyoshi; Shen, Guo Liang; Shikano, Sojin; Ritter, Robert; Zukas, Paul; Edelbaum, Dale; Morita, Akimichi; Takashima, Akira.

In: Journal of Biological Chemistry, Vol. 275, No. 16, 21.04.2000, p. 11957-11963.

Research output: Contribution to journalArticle

Ariizumi, K, Shen, GL, Shikano, S, Ritter, R, Zukas, P, Edelbaum, D, Morita, A & Takashima, A 2000, 'Cloning of a second dendritic cell-associated C-type lectin (dectin-2) and its alternatively spliced isoforms', Journal of Biological Chemistry, vol. 275, no. 16, pp. 11957-11963. https://doi.org/10.1074/jbc.275.16.11957
Ariizumi, Kiyoshi ; Shen, Guo Liang ; Shikano, Sojin ; Ritter, Robert ; Zukas, Paul ; Edelbaum, Dale ; Morita, Akimichi ; Takashima, Akira. / Cloning of a second dendritic cell-associated C-type lectin (dectin-2) and its alternatively spliced isoforms. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 16. pp. 11957-11963.
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