Cloning of an intrinsic human TFIID subunit that interacts with multiple transcriptional activators

Cheng Ming Chiang, Robert G. Roeder

Research output: Contribution to journalArticle

342 Citations (Scopus)

Abstract

TFIID is a multisubunit protein complex comprised of the TATA-binding protein (TBP) and multiple TBP-associated factors (TAFs). The TAFs in TEIID are essential for activator-dependent transcription. The cloning of a complementary DNA encoding a human TFIID TAF, TAFII55, that has no known homolog in Drosophila TFIID is now described. TAFII55 is shown to interact with the largest subunit (TAFII230) of human TFIID through its central region and with multiple activators-including Sp1, YY1, USE, CTF, adenoviral E1A, and human immunodeficiency virus-type 1 Tat proteins-through a distinct amino-terminal domain. The TAFII55-interacting region of Sp1 was localized to its DNA-binding domain, which is distinct from the glutamine-rich activation domains previously shown to interact with Drosophila TAF II110. Thus, this human TFIID TAF may be a co-activator that mediates a response to multiple activators through a distinct mechanism.

Original languageEnglish (US)
Pages (from-to)531-536
Number of pages6
JournalScience
Volume267
Issue number5197
StatePublished - 1995

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TATA-Binding Protein Associated Factors
Transcription Factor TFIID
Organism Cloning
Drosophila
tat Gene Products
TATA-Box Binding Protein
Glutamine
HIV-1
Complementary DNA
DNA
Proteins

ASJC Scopus subject areas

  • General

Cite this

Cloning of an intrinsic human TFIID subunit that interacts with multiple transcriptional activators. / Chiang, Cheng Ming; Roeder, Robert G.

In: Science, Vol. 267, No. 5197, 1995, p. 531-536.

Research output: Contribution to journalArticle

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