Cloning of human ovarian cancer cells in soft agar from malignant effusions and peritoneal washings

R. F. Ozols, J. K V Willson, K. R. Grotzinger, R. C. Young

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We have used a technique for the direct cloning in soft agar of human ovarian cancer cells to grow ovarian cancer colonies from 81% of malignant effusions obtained from 27 patients with ovarian cancer as well as from 92% of cytologically malignant peritoneal washings from 13 patients, collected in the absence of ascites. Ovarian cancer colonies were grown from washings obtained at peritoneoscopy or via an indwelling Tenckhoff dialysis catheter. No ovarian cancer colony growth was observed in cytologically negative peritoneal washings from 13 patients with ovarian cancer. Sufficient clonogenic malignant cells were collected by copious peritoneal lavage to allow for in vitro chemotherapy sensitivity testing using this same assay in three of six patients. These results indicate that the sensitivity of human ovarian cancer cells to antitumor agents can be determined even in patients without malignant effusions or readily biopsiable tumors if they have malignant peritoneal washings. Thirty separate in vitro chemotherapy sensitivity assays were performed using Adriamycin, cis-platinum, melphalan, and 5-fluorouracil. In previously treated patients, there were no instances of in vitro sensitivity using these agents at doses approximating clinically achievable plasma levels. However, in untreated patients, in vitro sensitivity to melphalan and Adriamycin was observed. These in vitro results correspond to clinically observed patterns of chemotherapy sensitivity in patients with ovarian cancer and provide indirect evidence that the stemcell assay is predictive for in vitro sensitivity to antitumor agents.

Original languageEnglish (US)
JournalCancer Research
Volume40
Issue number8
StatePublished - 1980

Fingerprint

Ascitic Fluid
Ovarian Neoplasms
Agar
Organism Cloning
Melphalan
Drug Therapy
Antineoplastic Agents
Doxorubicin
Peritoneal Lavage
Ascites
Fluorouracil
Laparoscopy
Cisplatin
In Vitro Techniques
Dialysis
Catheters
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ozols, R. F., Willson, J. K. V., Grotzinger, K. R., & Young, R. C. (1980). Cloning of human ovarian cancer cells in soft agar from malignant effusions and peritoneal washings. Cancer Research, 40(8).

Cloning of human ovarian cancer cells in soft agar from malignant effusions and peritoneal washings. / Ozols, R. F.; Willson, J. K V; Grotzinger, K. R.; Young, R. C.

In: Cancer Research, Vol. 40, No. 8, 1980.

Research output: Contribution to journalArticle

Ozols, RF, Willson, JKV, Grotzinger, KR & Young, RC 1980, 'Cloning of human ovarian cancer cells in soft agar from malignant effusions and peritoneal washings', Cancer Research, vol. 40, no. 8.
Ozols, R. F. ; Willson, J. K V ; Grotzinger, K. R. ; Young, R. C. / Cloning of human ovarian cancer cells in soft agar from malignant effusions and peritoneal washings. In: Cancer Research. 1980 ; Vol. 40, No. 8.
@article{907b05b257f14ecbb46c0d3fb3ca7160,
title = "Cloning of human ovarian cancer cells in soft agar from malignant effusions and peritoneal washings",
abstract = "We have used a technique for the direct cloning in soft agar of human ovarian cancer cells to grow ovarian cancer colonies from 81{\%} of malignant effusions obtained from 27 patients with ovarian cancer as well as from 92{\%} of cytologically malignant peritoneal washings from 13 patients, collected in the absence of ascites. Ovarian cancer colonies were grown from washings obtained at peritoneoscopy or via an indwelling Tenckhoff dialysis catheter. No ovarian cancer colony growth was observed in cytologically negative peritoneal washings from 13 patients with ovarian cancer. Sufficient clonogenic malignant cells were collected by copious peritoneal lavage to allow for in vitro chemotherapy sensitivity testing using this same assay in three of six patients. These results indicate that the sensitivity of human ovarian cancer cells to antitumor agents can be determined even in patients without malignant effusions or readily biopsiable tumors if they have malignant peritoneal washings. Thirty separate in vitro chemotherapy sensitivity assays were performed using Adriamycin, cis-platinum, melphalan, and 5-fluorouracil. In previously treated patients, there were no instances of in vitro sensitivity using these agents at doses approximating clinically achievable plasma levels. However, in untreated patients, in vitro sensitivity to melphalan and Adriamycin was observed. These in vitro results correspond to clinically observed patterns of chemotherapy sensitivity in patients with ovarian cancer and provide indirect evidence that the stemcell assay is predictive for in vitro sensitivity to antitumor agents.",
author = "Ozols, {R. F.} and Willson, {J. K V} and Grotzinger, {K. R.} and Young, {R. C.}",
year = "1980",
language = "English (US)",
volume = "40",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "8",

}

TY - JOUR

T1 - Cloning of human ovarian cancer cells in soft agar from malignant effusions and peritoneal washings

AU - Ozols, R. F.

AU - Willson, J. K V

AU - Grotzinger, K. R.

AU - Young, R. C.

PY - 1980

Y1 - 1980

N2 - We have used a technique for the direct cloning in soft agar of human ovarian cancer cells to grow ovarian cancer colonies from 81% of malignant effusions obtained from 27 patients with ovarian cancer as well as from 92% of cytologically malignant peritoneal washings from 13 patients, collected in the absence of ascites. Ovarian cancer colonies were grown from washings obtained at peritoneoscopy or via an indwelling Tenckhoff dialysis catheter. No ovarian cancer colony growth was observed in cytologically negative peritoneal washings from 13 patients with ovarian cancer. Sufficient clonogenic malignant cells were collected by copious peritoneal lavage to allow for in vitro chemotherapy sensitivity testing using this same assay in three of six patients. These results indicate that the sensitivity of human ovarian cancer cells to antitumor agents can be determined even in patients without malignant effusions or readily biopsiable tumors if they have malignant peritoneal washings. Thirty separate in vitro chemotherapy sensitivity assays were performed using Adriamycin, cis-platinum, melphalan, and 5-fluorouracil. In previously treated patients, there were no instances of in vitro sensitivity using these agents at doses approximating clinically achievable plasma levels. However, in untreated patients, in vitro sensitivity to melphalan and Adriamycin was observed. These in vitro results correspond to clinically observed patterns of chemotherapy sensitivity in patients with ovarian cancer and provide indirect evidence that the stemcell assay is predictive for in vitro sensitivity to antitumor agents.

AB - We have used a technique for the direct cloning in soft agar of human ovarian cancer cells to grow ovarian cancer colonies from 81% of malignant effusions obtained from 27 patients with ovarian cancer as well as from 92% of cytologically malignant peritoneal washings from 13 patients, collected in the absence of ascites. Ovarian cancer colonies were grown from washings obtained at peritoneoscopy or via an indwelling Tenckhoff dialysis catheter. No ovarian cancer colony growth was observed in cytologically negative peritoneal washings from 13 patients with ovarian cancer. Sufficient clonogenic malignant cells were collected by copious peritoneal lavage to allow for in vitro chemotherapy sensitivity testing using this same assay in three of six patients. These results indicate that the sensitivity of human ovarian cancer cells to antitumor agents can be determined even in patients without malignant effusions or readily biopsiable tumors if they have malignant peritoneal washings. Thirty separate in vitro chemotherapy sensitivity assays were performed using Adriamycin, cis-platinum, melphalan, and 5-fluorouracil. In previously treated patients, there were no instances of in vitro sensitivity using these agents at doses approximating clinically achievable plasma levels. However, in untreated patients, in vitro sensitivity to melphalan and Adriamycin was observed. These in vitro results correspond to clinically observed patterns of chemotherapy sensitivity in patients with ovarian cancer and provide indirect evidence that the stemcell assay is predictive for in vitro sensitivity to antitumor agents.

UR - http://www.scopus.com/inward/record.url?scp=0018819214&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018819214&partnerID=8YFLogxK

M3 - Article

VL - 40

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 8

ER -