Cloning of Human Ovarian Cancer Cells in Soft Agar from Malignant Effusions and Peritoneal Washings

Robert F. Ozols, James K V Willson, Karen R. Grotzinger, Robert C. Young

Research output: Contribution to journalArticle

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Abstract

We have used a technique for the direct cloning in soft agar of human ovarian cancer cells (Hamburger et al. Cancer Res., 38: 3438–3444, 1978) to grow ovarian cancer colonies from 81% of malignant effusions obtained from 27 patients with ovarian cancer as well as from 92% of cytologically malignant peritoneal washings from 13 patients, collected in the absence of ascites. Ovarian cancer colonies were grown from washings obtained at peritoneoscopy or via an indwelling Tenckhoff dialysis catheter. No ovarian cancer colony growth was observed in cytologically negative peritoneal washings from 13 patients with ovarian cancer. Sufficient clonogenic malignant cells were collected by copious peritoneal lavage to allow for in vitro chemotherapy sensitivity testing using this same assay in three of six patients. These results indicate that the sensitivity of human ovarian cancer cells to antitumor agents can be determined even in patients without malignant effusions or readily biopsiable tumors if they have malignant peritoneal washings. Thirty separate in vitro chemotherapy sensitivity assays were performed using Adriamycin, cis-platinum, melphalan, and 5-fluorouracil. In previously treated patients, there were no instances of in vitro sensitivity using these agents at doses approximating clinically achievable plasma levels. However, in untreated patients, in vitro sensitivity to melphalan and Adria-mycin was observed. These in vitro results correspond to clinically observed patterns of chemotherapy sensitivity in patients with ovarian cancer and provide indirect evidence that the stem cell assay is predictive for in vitro sensitivity to antitumor agents.

Original languageEnglish (US)
Pages (from-to)2743-2747
Number of pages5
JournalCancer Research
Volume40
StatePublished - Aug 1 1980

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Ascitic Fluid
Ovarian Neoplasms
Agar
Organism Cloning
Melphalan
Drug Therapy
Antineoplastic Agents
Colony-Forming Units Assay
Peritoneal Lavage
Ascites
Fluorouracil
Laparoscopy
Doxorubicin
Cisplatin
In Vitro Techniques
Dialysis
Neoplasms
Catheters
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ozols, R. F., Willson, J. K. V., Grotzinger, K. R., & Young, R. C. (1980). Cloning of Human Ovarian Cancer Cells in Soft Agar from Malignant Effusions and Peritoneal Washings. Cancer Research, 40, 2743-2747.

Cloning of Human Ovarian Cancer Cells in Soft Agar from Malignant Effusions and Peritoneal Washings. / Ozols, Robert F.; Willson, James K V; Grotzinger, Karen R.; Young, Robert C.

In: Cancer Research, Vol. 40, 01.08.1980, p. 2743-2747.

Research output: Contribution to journalArticle

Ozols, RF, Willson, JKV, Grotzinger, KR & Young, RC 1980, 'Cloning of Human Ovarian Cancer Cells in Soft Agar from Malignant Effusions and Peritoneal Washings', Cancer Research, vol. 40, pp. 2743-2747.
Ozols RF, Willson JKV, Grotzinger KR, Young RC. Cloning of Human Ovarian Cancer Cells in Soft Agar from Malignant Effusions and Peritoneal Washings. Cancer Research. 1980 Aug 1;40:2743-2747.
Ozols, Robert F. ; Willson, James K V ; Grotzinger, Karen R. ; Young, Robert C. / Cloning of Human Ovarian Cancer Cells in Soft Agar from Malignant Effusions and Peritoneal Washings. In: Cancer Research. 1980 ; Vol. 40. pp. 2743-2747.
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