Cloning, sequence, and tissue distribution of a rabbit renal Na+/H+ exchanger transcript

Friedhelm Hildebrandt; John H. Pizzonia; Robert F. Reilly; Nancy A. Rebouças; Claude Sardet; Jacques Pouysségur; Carolyn W. Slayman; Peter S. Aronson; Peter Igarashi

doi:10.1016/0167-4781(91)90221-7

Cloning, sequence, and tissue distribution of a rabbit renal Na⁺/H⁺ exchanger transcript

Friedhelm Hildebrandt, John H. Pizzonia, Robert F. Reilly, Nancy A. Rebouças, Claude Sardet, Jacques Pouysségur, Carolyn W. Slayman, Peter S. Aronson, Peter Igarashi

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Rabbit kidney expresses a transcript that is similar to the human growth-factor-activatable Na⁺/H⁺ exchanger. PCR and library screening were used to clone overlapping 2.5 kb, 1.4 kb, and 1.8 kb cDNAs that together contain the entire coding region (2448 bp) and 5′ untranslated region (726 bp) and part of the 3′ untranslated region (128 bp) of a rabbit renal Na⁺/H⁺ exchanger transcript. The nucleotide and inferred amino acid sequences are highly conserved between rabbit and human (88% nucleotide identity, 95% amino acid identity). In rabbit, the transcript is expressed in both epithelial and non-epithelial tissues, with highes expression in stomach, brain, kidney, lung and ileum, and minimal expression in liver and skeletal muscle.

Original language	English (US)
Pages (from-to)	105-108
Number of pages	4
Journal	BBA - Gene Structure and Expression
Volume	1129
Issue number	1
DOIs	https://doi.org/10.1016/0167-4781(91)90221-7
State	Published - Dec 2 1991

Keywords

(Rabbit kidney)
Amino acid sequence
Polymerase chain reaction
Sodium-proton exchange
cDNA cloning

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

Access to Document

10.1016/0167-4781(91)90221-7

Cite this

@article{35e6de14009449169ef02d2a2d9342a4,

title = "Cloning, sequence, and tissue distribution of a rabbit renal Na+/H+ exchanger transcript",

abstract = "Rabbit kidney expresses a transcript that is similar to the human growth-factor-activatable Na+/H+ exchanger. PCR and library screening were used to clone overlapping 2.5 kb, 1.4 kb, and 1.8 kb cDNAs that together contain the entire coding region (2448 bp) and 5′ untranslated region (726 bp) and part of the 3′ untranslated region (128 bp) of a rabbit renal Na+/H+ exchanger transcript. The nucleotide and inferred amino acid sequences are highly conserved between rabbit and human (88% nucleotide identity, 95% amino acid identity). In rabbit, the transcript is expressed in both epithelial and non-epithelial tissues, with highes expression in stomach, brain, kidney, lung and ileum, and minimal expression in liver and skeletal muscle.",

keywords = "(Rabbit kidney), Amino acid sequence, Polymerase chain reaction, Sodium-proton exchange, cDNA cloning",

author = "Friedhelm Hildebrandt and Pizzonia, {John H.} and Reilly, {Robert F.} and Rebou{\c c}as, {Nancy A.} and Claude Sardet and Jacques Pouyss{\'e}gur and Slayman, {Carolyn W.} and Aronson, {Peter S.} and Peter Igarashi",

note = "Funding Information: The technical assistance of Marianne Sadowski, Jen-nifer Elsemore, Thecla Abbiati, and Christine Shugrue is gratefully acknowledged. This work was supported by the Deutsche Forschungsgemeinschaft; American Heart Association Connecticut Affiliate, Inc.: Funda-~fio de Amparo h Pesquisa do Estado de Silo Paulo; and U.S. Public Health Service (grants DK-33793, DK-42921. AM-01423, DK-07259, and DK-01816).",

year = "1991",

month = dec,

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doi = "10.1016/0167-4781(91)90221-7",

language = "English (US)",

volume = "1129",

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T1 - Cloning, sequence, and tissue distribution of a rabbit renal Na+/H+ exchanger transcript

AU - Hildebrandt, Friedhelm

AU - Pizzonia, John H.

AU - Reilly, Robert F.

AU - Rebouças, Nancy A.

AU - Sardet, Claude

AU - Pouysségur, Jacques

AU - Slayman, Carolyn W.

AU - Aronson, Peter S.

AU - Igarashi, Peter

N1 - Funding Information: The technical assistance of Marianne Sadowski, Jen-nifer Elsemore, Thecla Abbiati, and Christine Shugrue is gratefully acknowledged. This work was supported by the Deutsche Forschungsgemeinschaft; American Heart Association Connecticut Affiliate, Inc.: Funda-~fio de Amparo h Pesquisa do Estado de Silo Paulo; and U.S. Public Health Service (grants DK-33793, DK-42921. AM-01423, DK-07259, and DK-01816).

PY - 1991/12/2

Y1 - 1991/12/2

N2 - Rabbit kidney expresses a transcript that is similar to the human growth-factor-activatable Na+/H+ exchanger. PCR and library screening were used to clone overlapping 2.5 kb, 1.4 kb, and 1.8 kb cDNAs that together contain the entire coding region (2448 bp) and 5′ untranslated region (726 bp) and part of the 3′ untranslated region (128 bp) of a rabbit renal Na+/H+ exchanger transcript. The nucleotide and inferred amino acid sequences are highly conserved between rabbit and human (88% nucleotide identity, 95% amino acid identity). In rabbit, the transcript is expressed in both epithelial and non-epithelial tissues, with highes expression in stomach, brain, kidney, lung and ileum, and minimal expression in liver and skeletal muscle.

AB - Rabbit kidney expresses a transcript that is similar to the human growth-factor-activatable Na+/H+ exchanger. PCR and library screening were used to clone overlapping 2.5 kb, 1.4 kb, and 1.8 kb cDNAs that together contain the entire coding region (2448 bp) and 5′ untranslated region (726 bp) and part of the 3′ untranslated region (128 bp) of a rabbit renal Na+/H+ exchanger transcript. The nucleotide and inferred amino acid sequences are highly conserved between rabbit and human (88% nucleotide identity, 95% amino acid identity). In rabbit, the transcript is expressed in both epithelial and non-epithelial tissues, with highes expression in stomach, brain, kidney, lung and ileum, and minimal expression in liver and skeletal muscle.

KW - (Rabbit kidney)

KW - Amino acid sequence

KW - Polymerase chain reaction

KW - Sodium-proton exchange

KW - cDNA cloning

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