Clostridium difficile antitoxin neutralization of cecal toxin(s) from guinea pigs with penicillin-associated colitis

J. E. Rehg, S. P. Pakes

Research output: Chapter in Book/Report/Conference proceedingChapter

19 Citations (Scopus)

Abstract

Within 3 to 5 days after an intramuscular injection of penicillin, a high percentage of guinea pigs died with an enterocolitis. Bacteria-free filtrates of cecal content from penicillin-treated guinea pigs were lethal for guinea pigs and mice. These filtrates also increased vascular permeability in the skin of rabbits and were cytopathic in the Y-1 adrenal cell tissue culture system. The tissue culture assay was the most sensitive of the bioassays for detecting the toxicity of the cecal filtrates. Clostridium difficile antitoxin neutralized all three biological effects, whereas Clostridium sordellii antitoxin did not prevent mice from dying when injected with a lethal dose of cecal filtrate. The neutralization patterns of the biological effects of these two antitoxins suggested Clostridium difficile toxin was responsible for the biological effects of the cecal filtrates. The neutralization patterns of these two antitoxins also indicated Clostridium difficile antitoxin should be used for definitive identification of Clostridium difficile toxin in cecal filtrates since Clostridium sordellii antitoxin did not neutralize all the biological effects of the cecal filtrates.

Original languageEnglish (US)
Title of host publicationLaboratory Animal Science
Pages156-160
Number of pages5
Volume31
Edition2
StatePublished - 1981

Fingerprint

Antitoxins
Clostridium difficile
Colitis
Penicillins
Guinea Pigs
Clostridium sordellii
Enterocolitis
Intramuscular Injections
Capillary Permeability
Biological Assay
Cell Culture Techniques
Rabbits
Bacteria
Skin

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Rehg, J. E., & Pakes, S. P. (1981). Clostridium difficile antitoxin neutralization of cecal toxin(s) from guinea pigs with penicillin-associated colitis. In Laboratory Animal Science (2 ed., Vol. 31, pp. 156-160)

Clostridium difficile antitoxin neutralization of cecal toxin(s) from guinea pigs with penicillin-associated colitis. / Rehg, J. E.; Pakes, S. P.

Laboratory Animal Science. Vol. 31 2. ed. 1981. p. 156-160.

Research output: Chapter in Book/Report/Conference proceedingChapter

Rehg, JE & Pakes, SP 1981, Clostridium difficile antitoxin neutralization of cecal toxin(s) from guinea pigs with penicillin-associated colitis. in Laboratory Animal Science. 2 edn, vol. 31, pp. 156-160.
Rehg JE, Pakes SP. Clostridium difficile antitoxin neutralization of cecal toxin(s) from guinea pigs with penicillin-associated colitis. In Laboratory Animal Science. 2 ed. Vol. 31. 1981. p. 156-160
Rehg, J. E. ; Pakes, S. P. / Clostridium difficile antitoxin neutralization of cecal toxin(s) from guinea pigs with penicillin-associated colitis. Laboratory Animal Science. Vol. 31 2. ed. 1981. pp. 156-160
@inbook{c2320f36f4e4444cb3d9193d320d6efa,
title = "Clostridium difficile antitoxin neutralization of cecal toxin(s) from guinea pigs with penicillin-associated colitis",
abstract = "Within 3 to 5 days after an intramuscular injection of penicillin, a high percentage of guinea pigs died with an enterocolitis. Bacteria-free filtrates of cecal content from penicillin-treated guinea pigs were lethal for guinea pigs and mice. These filtrates also increased vascular permeability in the skin of rabbits and were cytopathic in the Y-1 adrenal cell tissue culture system. The tissue culture assay was the most sensitive of the bioassays for detecting the toxicity of the cecal filtrates. Clostridium difficile antitoxin neutralized all three biological effects, whereas Clostridium sordellii antitoxin did not prevent mice from dying when injected with a lethal dose of cecal filtrate. The neutralization patterns of the biological effects of these two antitoxins suggested Clostridium difficile toxin was responsible for the biological effects of the cecal filtrates. The neutralization patterns of these two antitoxins also indicated Clostridium difficile antitoxin should be used for definitive identification of Clostridium difficile toxin in cecal filtrates since Clostridium sordellii antitoxin did not neutralize all the biological effects of the cecal filtrates.",
author = "Rehg, {J. E.} and Pakes, {S. P.}",
year = "1981",
language = "English (US)",
volume = "31",
pages = "156--160",
booktitle = "Laboratory Animal Science",
edition = "2",

}

TY - CHAP

T1 - Clostridium difficile antitoxin neutralization of cecal toxin(s) from guinea pigs with penicillin-associated colitis

AU - Rehg, J. E.

AU - Pakes, S. P.

PY - 1981

Y1 - 1981

N2 - Within 3 to 5 days after an intramuscular injection of penicillin, a high percentage of guinea pigs died with an enterocolitis. Bacteria-free filtrates of cecal content from penicillin-treated guinea pigs were lethal for guinea pigs and mice. These filtrates also increased vascular permeability in the skin of rabbits and were cytopathic in the Y-1 adrenal cell tissue culture system. The tissue culture assay was the most sensitive of the bioassays for detecting the toxicity of the cecal filtrates. Clostridium difficile antitoxin neutralized all three biological effects, whereas Clostridium sordellii antitoxin did not prevent mice from dying when injected with a lethal dose of cecal filtrate. The neutralization patterns of the biological effects of these two antitoxins suggested Clostridium difficile toxin was responsible for the biological effects of the cecal filtrates. The neutralization patterns of these two antitoxins also indicated Clostridium difficile antitoxin should be used for definitive identification of Clostridium difficile toxin in cecal filtrates since Clostridium sordellii antitoxin did not neutralize all the biological effects of the cecal filtrates.

AB - Within 3 to 5 days after an intramuscular injection of penicillin, a high percentage of guinea pigs died with an enterocolitis. Bacteria-free filtrates of cecal content from penicillin-treated guinea pigs were lethal for guinea pigs and mice. These filtrates also increased vascular permeability in the skin of rabbits and were cytopathic in the Y-1 adrenal cell tissue culture system. The tissue culture assay was the most sensitive of the bioassays for detecting the toxicity of the cecal filtrates. Clostridium difficile antitoxin neutralized all three biological effects, whereas Clostridium sordellii antitoxin did not prevent mice from dying when injected with a lethal dose of cecal filtrate. The neutralization patterns of the biological effects of these two antitoxins suggested Clostridium difficile toxin was responsible for the biological effects of the cecal filtrates. The neutralization patterns of these two antitoxins also indicated Clostridium difficile antitoxin should be used for definitive identification of Clostridium difficile toxin in cecal filtrates since Clostridium sordellii antitoxin did not neutralize all the biological effects of the cecal filtrates.

UR - http://www.scopus.com/inward/record.url?scp=0019797421&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019797421&partnerID=8YFLogxK

M3 - Chapter

VL - 31

SP - 156

EP - 160

BT - Laboratory Animal Science

ER -