Abstract
Within 3 to 5 days after an intramuscular injection of penicillin, a high percentage of guinea pigs died with an enterocolitis. Bacteria-free filtrates of cecal content from penicillin-treated guinea pigs were lethal for guinea pigs and mice. These filtrates also increased vascular permeability in the skin of rabbits and were cytopathic in the Y-1 adrenal cell tissue culture system. The tissue culture assay was the most sensitive of the bioassays for detecting the toxicity of the cecal filtrates. Clostridium difficile antitoxin neutralized all three biological effects, whereas Clostridium sordellii antitoxin did not prevent mice from dying when injected with a lethal dose of cecal filtrate. The neutralization patterns of the biological effects of these two antitoxins suggested Clostridium difficile toxin was responsible for the biological effects of the cecal filtrates. The neutralization patterns of these two antitoxins also indicated Clostridium difficile antitoxin should be used for definitive identification of Clostridium difficile toxin in cecal filtrates since Clostridium sordellii antitoxin did not neutralize all the biological effects of the cecal filtrates.
| Original language | English (US) |
|---|---|
| Title of host publication | Laboratory Animal Science |
| Pages | 156-160 |
| Number of pages | 5 |
| Volume | 31 |
| Edition | 2 |
| State | Published - 1981 |
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ASJC Scopus subject areas
- Medicine(all)
Cite this
Clostridium difficile antitoxin neutralization of cecal toxin(s) from guinea pigs with penicillin-associated colitis. / Rehg, J. E.; Pakes, S. P.
Laboratory Animal Science. Vol. 31 2. ed. 1981. p. 156-160.Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Clostridium difficile antitoxin neutralization of cecal toxin(s) from guinea pigs with penicillin-associated colitis
AU - Rehg, J. E.
AU - Pakes, S. P.
PY - 1981
Y1 - 1981
N2 - Within 3 to 5 days after an intramuscular injection of penicillin, a high percentage of guinea pigs died with an enterocolitis. Bacteria-free filtrates of cecal content from penicillin-treated guinea pigs were lethal for guinea pigs and mice. These filtrates also increased vascular permeability in the skin of rabbits and were cytopathic in the Y-1 adrenal cell tissue culture system. The tissue culture assay was the most sensitive of the bioassays for detecting the toxicity of the cecal filtrates. Clostridium difficile antitoxin neutralized all three biological effects, whereas Clostridium sordellii antitoxin did not prevent mice from dying when injected with a lethal dose of cecal filtrate. The neutralization patterns of the biological effects of these two antitoxins suggested Clostridium difficile toxin was responsible for the biological effects of the cecal filtrates. The neutralization patterns of these two antitoxins also indicated Clostridium difficile antitoxin should be used for definitive identification of Clostridium difficile toxin in cecal filtrates since Clostridium sordellii antitoxin did not neutralize all the biological effects of the cecal filtrates.
AB - Within 3 to 5 days after an intramuscular injection of penicillin, a high percentage of guinea pigs died with an enterocolitis. Bacteria-free filtrates of cecal content from penicillin-treated guinea pigs were lethal for guinea pigs and mice. These filtrates also increased vascular permeability in the skin of rabbits and were cytopathic in the Y-1 adrenal cell tissue culture system. The tissue culture assay was the most sensitive of the bioassays for detecting the toxicity of the cecal filtrates. Clostridium difficile antitoxin neutralized all three biological effects, whereas Clostridium sordellii antitoxin did not prevent mice from dying when injected with a lethal dose of cecal filtrate. The neutralization patterns of the biological effects of these two antitoxins suggested Clostridium difficile toxin was responsible for the biological effects of the cecal filtrates. The neutralization patterns of these two antitoxins also indicated Clostridium difficile antitoxin should be used for definitive identification of Clostridium difficile toxin in cecal filtrates since Clostridium sordellii antitoxin did not neutralize all the biological effects of the cecal filtrates.
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UR - http://www.scopus.com/inward/citedby.url?scp=0019797421&partnerID=8YFLogxK
M3 - Chapter
C2 - 7242013
AN - SCOPUS:0019797421
VL - 31
SP - 156
EP - 160
BT - Laboratory Animal Science
ER -