Clues to the mechanism of cholesterol transfer from the structure of NPC1 middle lumenal domain bound to NPC2

Xiaochun Li, Piyali Saha, Jian Lib, Günter Blobel, Suzanne R. Pfeffer

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Export of LDL-derived cholesterol from lysosomes requires the cooperation of the integral membrane protein Niemann-Pick C1 (NPC1) and a soluble protein, Niemann-Pick C2 (NPC2). Mutations in the genes encoding these proteins lead to Niemann-Pick disease type C (NPC). NPC2 binds to NPC1's second (middle), lumenally oriented domain (MLD) and transfers cholesterol to NPC1's N-terminal domain (NTD). Here, we report the 2.4-Å resolution crystal structure of a complex of human NPC1-MLD and NPC2 bearing bound cholesterol-3-O-sulfate. NPC1-MLD uses two protruding loops to bind NPC2, analogous to its interaction with the primed Ebola virus glycoprotein. Docking of the NPC1-NPC2 complex onto the full-length NPC1 structure reveals a direct cholesterol transfer tunnel between NPC2 and NTD cholesterol binding pockets, supporting the "hydrophobic hand-off" cholesterol transfer model.

Original languageEnglish (US)
Pages (from-to)10079-10084
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number36
DOIs
StatePublished - Sep 6 2016

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Cholesterol
Type C Niemann-Pick Disease
Ebolavirus
Lysosomes
LDL Cholesterol
Sulfates
Glycoproteins
Membrane Proteins
Proteins
Hand
Mutation

Keywords

  • Cholesterol trafficking
  • Crystal structure.
  • Ebola virus glycoprotein
  • Niemann-Pick type C disease

ASJC Scopus subject areas

  • General

Cite this

Clues to the mechanism of cholesterol transfer from the structure of NPC1 middle lumenal domain bound to NPC2. / Li, Xiaochun; Saha, Piyali; Lib, Jian; Blobel, Günter; Pfeffer, Suzanne R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, No. 36, 06.09.2016, p. 10079-10084.

Research output: Contribution to journalArticle

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abstract = "Export of LDL-derived cholesterol from lysosomes requires the cooperation of the integral membrane protein Niemann-Pick C1 (NPC1) and a soluble protein, Niemann-Pick C2 (NPC2). Mutations in the genes encoding these proteins lead to Niemann-Pick disease type C (NPC). NPC2 binds to NPC1's second (middle), lumenally oriented domain (MLD) and transfers cholesterol to NPC1's N-terminal domain (NTD). Here, we report the 2.4-{\AA} resolution crystal structure of a complex of human NPC1-MLD and NPC2 bearing bound cholesterol-3-O-sulfate. NPC1-MLD uses two protruding loops to bind NPC2, analogous to its interaction with the primed Ebola virus glycoprotein. Docking of the NPC1-NPC2 complex onto the full-length NPC1 structure reveals a direct cholesterol transfer tunnel between NPC2 and NTD cholesterol binding pockets, supporting the {"}hydrophobic hand-off{"} cholesterol transfer model.",
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