Co-immunoprecipitation and semi-quantitative immunoblotting for the analysis of protein-protein interactions

Christoph Jakob Burckhardt, John D. Minna, Gaudenz Danuser

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Co-immunoprecipitation (co-IP) of protein complexes from cell lysates is widely used to study protein-protein interactions. However, establishing robust co-IP assays often involves considerable optimization. Moreover, co-IP results are frequently presented in non-quantitative ways. This protocol presents an optimized co-IP workflow with an analysis based on semi-quantitative immunoblot densitometry to increase reliability and reproducibility. For complete details on the use and execution of this protocol, please refer to Burckhardt et al. (2021).

Original languageEnglish (US)
Article number100644
JournalSTAR Protocols
Volume2
Issue number3
DOIs
StatePublished - Sep 17 2021

Keywords

  • Cell Biology
  • Gene Expression
  • In Situ Hybridization
  • Microscopy
  • Model Organisms
  • Molecular Biology
  • Single Cell
  • Single-molecule Assays

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

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