Coated-vesicle formation in vitro: conflicting results using different assays

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Cell-free systems provide essential tools for elucidating the molecular mechanisms underlying complex cellular processes such as vesicular transport. The biochemical utility of these model systems is strengthened by assays that allow rapid, quantitative detection of the events being studied. Two model systems have recently been developed to reconstitute coated-vesicle budding, and two different biochemical assays are used to detect this event. Striking differences in the biochemical requirements for 'coated-vesicle budding' are detected by these two assays, suggesting that two distinct events are being measured. These findings have wide implications for the use of cell-free assay systems in cell biology.

Original languageEnglish (US)
Pages (from-to)145-148
Number of pages4
JournalTrends in Cell Biology
Volume3
Issue number5
DOIs
StatePublished - 1993

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Coated Vesicles
Cell-Free System
Cell Biology
In Vitro Techniques

ASJC Scopus subject areas

  • Cell Biology

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Coated-vesicle formation in vitro : conflicting results using different assays. / Schmid, Sandra L.

In: Trends in Cell Biology, Vol. 3, No. 5, 1993, p. 145-148.

Research output: Contribution to journalArticle

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