Coculture of Staphylococcus aureus with Pseudomonas aeruginosa drives S. aureus towards fermentative metabolism and reduced viability in a cystic fibrosis model

Laura M. Filkins, Jyoti A. Graber, Daniel G. Olson, Emily L. Dolben, Lee R. Lynd, Sabin Bhuju, George A. O'Toole

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

The airways of patients with cystic fibrosis are colonized with diverse bacterial communities that change dynamically during pediatric years and early adulthood. Staphylococcus aureus is the most prevalent pathogen during early childhood, but during late teens and early adulthood, a shift in microbial composition occurs leading to Pseudomonas aeruginosa community predominance in ~50% of adults. We developed a robust dual-bacterial in vitro coculture system of P. aeruginosa and S. aureus on monolayers of human bronchial epithelial cells homozygous for the ΔF508 cystic fibrosis transmembrane conductance regulator (CFTR) mutation to better model the mechanisms of this interaction. We show that P. aeruginosa drives the S. aureus expression profile from that of aerobic respiration to fermentation. This shift is dependent on the production of both 2-heptyl-4-hydroxyquinoline N-oxide (HQNO) and siderophores by P. aeruginosa. Furthermore, S. aureus-produced lactate is a carbon source that P. aeruginosa preferentially consumes over medium-supplied glucose. We find that initially S. aureus and P. aeruginosa coexist; however, over extended coculture P. aeruginosa reduces S. aureus viability, also in an HQNO- and P. aeruginosa siderophore-dependent manner. Interestingly, S. aureus small-colony-variant (SCV) genetic mutant strains, which have defects in their electron transport chain, experience reduced killing by P. aeruginosa compared to their wild-type parent strains; thus, SCVs may provide a mechanism for persistence of S. aureus in the presence of P. aeruginosa. We propose that the mechanism of P. aeruginosa-mediated killing of S. aureus is multifactorial, requiring HQNO and P. aeruginosa siderophores as well as additional genetic, environmental, and nutritional factors.

Original languageEnglish (US)
Pages (from-to)2252-2264
Number of pages13
JournalJournal of Bacteriology
Volume197
Issue number14
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

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Coculture Techniques
Cystic Fibrosis
Pseudomonas aeruginosa
Staphylococcus aureus
Siderophores
Nutrigenomics
Cystic Fibrosis Transmembrane Conductance Regulator
Electron Transport
Fermentation
Lactic Acid
Respiration
Carbon
Epithelial Cells
Pediatrics
Glucose

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Coculture of Staphylococcus aureus with Pseudomonas aeruginosa drives S. aureus towards fermentative metabolism and reduced viability in a cystic fibrosis model. / Filkins, Laura M.; Graber, Jyoti A.; Olson, Daniel G.; Dolben, Emily L.; Lynd, Lee R.; Bhuju, Sabin; O'Toole, George A.

In: Journal of Bacteriology, Vol. 197, No. 14, 01.01.2015, p. 2252-2264.

Research output: Contribution to journalArticle

Filkins, Laura M. ; Graber, Jyoti A. ; Olson, Daniel G. ; Dolben, Emily L. ; Lynd, Lee R. ; Bhuju, Sabin ; O'Toole, George A. / Coculture of Staphylococcus aureus with Pseudomonas aeruginosa drives S. aureus towards fermentative metabolism and reduced viability in a cystic fibrosis model. In: Journal of Bacteriology. 2015 ; Vol. 197, No. 14. pp. 2252-2264.
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abstract = "The airways of patients with cystic fibrosis are colonized with diverse bacterial communities that change dynamically during pediatric years and early adulthood. Staphylococcus aureus is the most prevalent pathogen during early childhood, but during late teens and early adulthood, a shift in microbial composition occurs leading to Pseudomonas aeruginosa community predominance in ~50{\%} of adults. We developed a robust dual-bacterial in vitro coculture system of P. aeruginosa and S. aureus on monolayers of human bronchial epithelial cells homozygous for the ΔF508 cystic fibrosis transmembrane conductance regulator (CFTR) mutation to better model the mechanisms of this interaction. We show that P. aeruginosa drives the S. aureus expression profile from that of aerobic respiration to fermentation. This shift is dependent on the production of both 2-heptyl-4-hydroxyquinoline N-oxide (HQNO) and siderophores by P. aeruginosa. Furthermore, S. aureus-produced lactate is a carbon source that P. aeruginosa preferentially consumes over medium-supplied glucose. We find that initially S. aureus and P. aeruginosa coexist; however, over extended coculture P. aeruginosa reduces S. aureus viability, also in an HQNO- and P. aeruginosa siderophore-dependent manner. Interestingly, S. aureus small-colony-variant (SCV) genetic mutant strains, which have defects in their electron transport chain, experience reduced killing by P. aeruginosa compared to their wild-type parent strains; thus, SCVs may provide a mechanism for persistence of S. aureus in the presence of P. aeruginosa. We propose that the mechanism of P. aeruginosa-mediated killing of S. aureus is multifactorial, requiring HQNO and P. aeruginosa siderophores as well as additional genetic, environmental, and nutritional factors.",
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