Codon usage biases co-evolve with transcription termination machinery to suppress premature cleavage and polyadenylation

Zhipeng Zhou, Yunkun Dang, Mian Zhou, Haiyan Yuan, Yi Liu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Codon usage biases are found in all genomes and influence protein expression levels. The codon usage effect on protein expression was thought to be mainly due to its impact on translation. Here, we show that transcription termination is an important driving force for codon usage bias in eukaryotes. Using Neurospora crassa as a model organism, we demonstrated that introduction of rare codons results in premature transcription termination (PTT) within open reading frames and abolishment of full-length mRNA. PTT is a wide-spread phenomenon in Neurospora, and there is a strong negative correlation between codon usage bias and PTT events. Rare codons lead to the formation of putative poly(A) signals and PTT. A similar role for codon usage bias was also observed in mouse cells. Together, these results suggest that codon usage biases co-evolve with the transcription termination machinery to suppress premature termination of transcription and thus allow for optimal gene expression.

Original languageEnglish (US)
Article numbere33569
JournaleLife
Volume7
DOIs
StatePublished - Mar 16 2018

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Polyadenylation
Transcription
Codon
Machinery
Poly A
Neurospora
Neurospora crassa
Gene expression
Eukaryota
Proteins
Open Reading Frames
Genes
Genome
Messenger RNA
Gene Expression

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Codon usage biases co-evolve with transcription termination machinery to suppress premature cleavage and polyadenylation. / Zhou, Zhipeng; Dang, Yunkun; Zhou, Mian; Yuan, Haiyan; Liu, Yi.

In: eLife, Vol. 7, e33569, 16.03.2018.

Research output: Contribution to journalArticle

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