Collaboration between macrophages and vaccine-induced CD4+ T cells confers protection against lethal pseudomonas aeruginosa pneumonia during neutropenia

Akinobu Kamei, Weihui Wu, David C. Traficante, Andrew Y. Koh, Nico Van Rooijen, Gerald B. Pier, Gregory P. Priebe

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The usefulness of vaccine-based strategies to prevent lethal bacterial infection in a host with neutropenia is not well-defined. Here, we show in a neutropenic mouse model that immunity induced by mucosal vaccination with a live-attenuated Pseudomonas aeruginosa vaccine is protective against lethal P. aeruginosa pneumonia caused by both vaccine-homologous and vaccine-heterologous strains, whereas passive immunization confers only vaccine-homologous protection. Cells in the macrophage lineage served as crucial innate cellular effectors in the neutropenic host after active immunization. Vaccine efficacy was CD4+ T-cell dependent and associated with accumulation of macrophage-lineage cells in the alveolar space after infection, as well as with enhanced P. aeruginosa clearance from the lung. Adaptive CD4+ T cells produced granulocyte-macrophage colony-stimulating factor (GM-CSF) on restimulation in vitro, and local GM-CSF was critical for vaccine efficacy. Thus, collaboration between the innate and adaptive effectors induced by mucosal vaccination can overcome neutropenia and confer protection against lethal bacterial infection in the profoundly neutropenic host.

Original languageEnglish (US)
Pages (from-to)39-49
Number of pages11
JournalJournal of Infectious Diseases
Volume207
Issue number1
DOIs
StatePublished - Jan 1 2013

Fingerprint

Cytoprotection
Neutropenia
Pseudomonas aeruginosa
Pneumonia
Vaccines
Macrophages
T-Lymphocytes
Vaccination
Granulocyte-Macrophage Colony-Stimulating Factor
Bacterial Infections
Pseudomonas Vaccines
Alveolar Epithelial Cells
Mucosal Immunity
Attenuated Vaccines
Passive Immunization
Lung
Infection

Keywords

  • CD4
  • GM-CSF
  • Immunocompromise
  • Infection
  • Macrophage
  • Monocyte
  • Neutropenia
  • Pneumonia
  • Pseudomonas aeruginosa
  • Vaccines

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

Cite this

Collaboration between macrophages and vaccine-induced CD4+ T cells confers protection against lethal pseudomonas aeruginosa pneumonia during neutropenia. / Kamei, Akinobu; Wu, Weihui; Traficante, David C.; Koh, Andrew Y.; Van Rooijen, Nico; Pier, Gerald B.; Priebe, Gregory P.

In: Journal of Infectious Diseases, Vol. 207, No. 1, 01.01.2013, p. 39-49.

Research output: Contribution to journalArticle

Kamei, Akinobu ; Wu, Weihui ; Traficante, David C. ; Koh, Andrew Y. ; Van Rooijen, Nico ; Pier, Gerald B. ; Priebe, Gregory P. / Collaboration between macrophages and vaccine-induced CD4+ T cells confers protection against lethal pseudomonas aeruginosa pneumonia during neutropenia. In: Journal of Infectious Diseases. 2013 ; Vol. 207, No. 1. pp. 39-49.
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