Collagen fibril flow and tissue translocation coupled to fibroblast migration in 3D collagen matrices

Miguel Miron-Mendoza, Joachim Seemann, Frederick Grinnell

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

In nested collagen matrices, human fibroblasts migrate from cell-containing dermal equivalents into surrounding cell-free outer matrices. Time-lapse microscopy showed that in addition to cell migration, collagen fibril flow occurred in the outer matrix toward the interface with the dermal equivalent. Features of this flow suggested that it depends on the same cell motile machinery that normally results in cell migration. Collagen fibril flow was capable of producing large-scale tissue translocation as shown by closure of a ∼1-mm gap between paired dermal equivalents in floating, nested collagen matrices. Our findings demonstrate that when fibroblasts interact with collagen matrices, tractional force exerted by the cells can couple to matrix translocation as well as to cell migration.

Original languageEnglish (US)
Pages (from-to)2051-2058
Number of pages8
JournalMolecular Biology of the Cell
Volume19
Issue number5
DOIs
StatePublished - May 2008

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Collagen
Fibroblasts
Cell Movement
Skin
Microscopy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Collagen fibril flow and tissue translocation coupled to fibroblast migration in 3D collagen matrices. / Miron-Mendoza, Miguel; Seemann, Joachim; Grinnell, Frederick.

In: Molecular Biology of the Cell, Vol. 19, No. 5, 05.2008, p. 2051-2058.

Research output: Contribution to journalArticle

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