Collecting duct-specific gene inactivation of αENaC in the mouse kidney does not impair sodium and potassium balance

Isabelle Rubera, Johannes Loffing, Lawrence G. Palmer, Gustavo Frindt, Nicole Fowler-Jaeger, Daniel Saurer, Tom Carroll, Andrew McMahon, Edith Hummler, Bernard C. Rossier

Research output: Contribution to journalArticle

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Abstract

Aldosterone controls the final sodium reabsorption and potassium secretion in the kidney by regulating the activity of the epithelial sodium channel (ENaC) in the aldosterone-sensitive distal nephron (ASDN). ASDN consists of the last portion of the distal convoluted tubule (late DCT), the connecting tubule (CNT), and the collecting duct (CD) (i.e., the cortical CD [CCD] and the medullary CD [MCD]). It has been proposed that the control of sodium transport in the CCD is essential for achieving sodium and potassium balance. We have tested this hypothesis by inactivating the α subunit of ENaC in the CD but leaving ENaC expression in the late DCT and CNT intact. Under salt restriction or under aldosterone infusion, whole-cell voltage clamp of principal cells of CCD showed no detectable ENaC activity, whereas large amiloride-sensitive currents were observed in control littermates. The animals survive well and are able to maintain sodium and potassium balance, even when challenged by salt restriction, water deprivation, or potassium loading. We conclude that the expression of ENaC in the CD is not a prerequisite for achieving sodium and potassium balance in mice. This stresses the importance of more proximal nephron segments (late DCT/CNT) to achieve sodium and potassium balance.

Original languageEnglish (US)
Pages (from-to)554-565
Number of pages12
JournalJournal of Clinical Investigation
Volume112
Issue number4
DOIs
StatePublished - Jan 1 2003

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Gene Silencing
Potassium
Sodium
Aldosterone
Kidney
Nephrons
Salts
Epithelial Sodium Channels
Water Deprivation
Amiloride

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Rubera, I., Loffing, J., Palmer, L. G., Frindt, G., Fowler-Jaeger, N., Saurer, D., ... Rossier, B. C. (2003). Collecting duct-specific gene inactivation of αENaC in the mouse kidney does not impair sodium and potassium balance. Journal of Clinical Investigation, 112(4), 554-565. https://doi.org/10.1172/JCI16956

Collecting duct-specific gene inactivation of αENaC in the mouse kidney does not impair sodium and potassium balance. / Rubera, Isabelle; Loffing, Johannes; Palmer, Lawrence G.; Frindt, Gustavo; Fowler-Jaeger, Nicole; Saurer, Daniel; Carroll, Tom; McMahon, Andrew; Hummler, Edith; Rossier, Bernard C.

In: Journal of Clinical Investigation, Vol. 112, No. 4, 01.01.2003, p. 554-565.

Research output: Contribution to journalArticle

Rubera, I, Loffing, J, Palmer, LG, Frindt, G, Fowler-Jaeger, N, Saurer, D, Carroll, T, McMahon, A, Hummler, E & Rossier, BC 2003, 'Collecting duct-specific gene inactivation of αENaC in the mouse kidney does not impair sodium and potassium balance', Journal of Clinical Investigation, vol. 112, no. 4, pp. 554-565. https://doi.org/10.1172/JCI16956
Rubera, Isabelle ; Loffing, Johannes ; Palmer, Lawrence G. ; Frindt, Gustavo ; Fowler-Jaeger, Nicole ; Saurer, Daniel ; Carroll, Tom ; McMahon, Andrew ; Hummler, Edith ; Rossier, Bernard C. / Collecting duct-specific gene inactivation of αENaC in the mouse kidney does not impair sodium and potassium balance. In: Journal of Clinical Investigation. 2003 ; Vol. 112, No. 4. pp. 554-565.
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