Colocalization of ghrelin O-acyltransferase and ghrelin in gastric mucosal cells

Ichiro Sakata, Jing Yang, Charlotte E. Lee, Sherri Osborne-Lawrence, Sherry A. Rovinsky, Joel K. Elmquist, Jeffrey M. Zigman

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Ghrelin is a peptide hormone with many known functions, including orexigenic, blood glucose-regulatory, and antidepressant actions, among others. Mature ghrelin is unique in that it is the only known naturally occurring peptide to be posttranslationally modified by O-acylation with octanoate. This acylation is required for many of ghrelin's actions, including its effects on promoting increases in food intake and body weight. GOAT (ghrelin O-acyltransferase), one of 16 members of the MBOAT family of membrane-bound O-acyltransferases, has recently been identified as the enzyme responsible for catalyzing the addition of the octanoyl group to ghrelin. Although the initial reports of GOAT have localized its encoding mRNA to tissues known to contain ghrelin, it is as yet unclear whether the octanoylation occurs within ghrelin-producing cells or in neighboring cells. Here, we have performed dual-label histochemical analysis on mouse stomach sections and quantitative PCR on mRNAs from highly enriched pools of mouse gastric ghrelin cells to demonstrate a high degree of GOAT mRNA expression within ghrelin-producing cells of the gastric oxyntic mucosa. We also demonstrate that GOAT is the only member of the MBOAT family whose expression is highly enriched within gastric ghrelin cells and whose whole body distribution mirrors that of ghrelin.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume297
Issue number1
DOIs
StatePublished - Jul 2009

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Acyltransferases
Ghrelin
Stomach
Acylation
Messenger RNA
Gastric Parietal Cells
Peptide Hormones
Gastric Mucosa
Antidepressive Agents

Keywords

  • Membrane-bound O-acyltransferases
  • Octanoate

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Endocrinology, Diabetes and Metabolism

Cite this

Colocalization of ghrelin O-acyltransferase and ghrelin in gastric mucosal cells. / Sakata, Ichiro; Yang, Jing; Lee, Charlotte E.; Osborne-Lawrence, Sherri; Rovinsky, Sherry A.; Elmquist, Joel K.; Zigman, Jeffrey M.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 297, No. 1, 07.2009.

Research output: Contribution to journalArticle

Sakata, Ichiro ; Yang, Jing ; Lee, Charlotte E. ; Osborne-Lawrence, Sherri ; Rovinsky, Sherry A. ; Elmquist, Joel K. ; Zigman, Jeffrey M. / Colocalization of ghrelin O-acyltransferase and ghrelin in gastric mucosal cells. In: American Journal of Physiology - Endocrinology and Metabolism. 2009 ; Vol. 297, No. 1.
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