Colon cancer secreted protein-2 (CCSP-2), a novel candidate serological marker of colon neoplasia

Baozhong Xin, Petra Platzer, Stephen P. Fink, Lisa Reese, Arman Nosrati, James K V Willson, Keith Wilson, Sanford Markowitz

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Cancers of the colon and rectum are the second leading cause of cancer death among adult Americans. When detected at early stages, colon cancer is highly curable. Colonoscopy, an effective but invasive screening test, has been limited in its public acceptance. The goal of this study was to identify novel serum markers of colon cancers and precancerous colon adenomas as potential candidates for noninvasive detection of early colon neoplasms. Employing expression microarrays, we identified colon cancer secreted protein-2 (CCSP-2) as a novel transcript whose expression is generally absent in normal colon and other normal body tissues, but that is induced an average of 78-fold in Stage II, III, and IV colon cancers, as well as in colon adenomas and colon cancer cell lines. These findings were validated by real-time PCR analysis in an independent panel of colon cancer cases. Moreover, CCSP-2 was shown to encode a secreted protein that circulates stably and is detectable in the blood of mice bearing human cancer xenografts transfected with epitope-tagged CCSP-2. As a novel secreted protein that is markedly induced in colon adenomas and cancers, CCSP-2 is a novel candidate for development as a diagnostic serum marker of early stage colon cancer.

Original languageEnglish (US)
Pages (from-to)724-731
Number of pages8
JournalOncogene
Volume24
Issue number4
DOIs
StatePublished - Jan 20 2005

Keywords

  • Colon cancer
  • Secreted proteins
  • Serum markers

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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    Xin, B., Platzer, P., Fink, S. P., Reese, L., Nosrati, A., Willson, J. K. V., Wilson, K., & Markowitz, S. (2005). Colon cancer secreted protein-2 (CCSP-2), a novel candidate serological marker of colon neoplasia. Oncogene, 24(4), 724-731. https://doi.org/10.1038/sj.onc.1208134