Commensal-associated molecular patterns induce selective toll-like receptor-trafficking from apical membrane to cytoplasmic compartments in polarized intestinal epithelium

Elke Cario, Dennis Brown, Mary McKee, Kathryn Lynch-Devaney, Guido Gerken, Daniel K. Podolsky

Research output: Contribution to journalArticle

235 Citations (Scopus)

Abstract

Commensal-associated molecular patterns, the major products of nonpathogenic bacteria, are present at high concentrations at the apical surface of the intestinal epithelium. However, the nature of the interaction of commensal-associated molecular patterns with the lumenal surface of the epithelium has not been defined. We have recently demonstrated that intestinal epithelial cells constitutively express several Toll-like receptors (TLRs) in vitro and in vivo that seem to be the key receptors responsible for immune cell activation in response to various bacterial products. In this study we characterize the subcellular distribution of two major TLRs, TLR2 and TLR4, and their ligand-specific dynamic regulation in the model human intestinal epithelial cell line T84. Immunocytochemical studies indicate that TLR2 and TLR4 are constitutively expressed at the apical pole of differentiated T84 cells. After stimulation with lipopolysaccharide or peptidoglycan, TLRs selectively traffic to cytoplasmic compartments near the basolateral membrane. Thus, we demonstrate that TLRs are positioned at the apical pole where they are poised to monitor the sensitive balance of the lumenal microbial array. The results of this dynamic epithelial surveillance can then be conveyed to the underlying cell populations of the lamina propria via these innate immune pattern recognition receptors.

Original languageEnglish (US)
Pages (from-to)165-173
Number of pages9
JournalAmerican Journal of Pathology
Volume160
Issue number1
StatePublished - 2002

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Toll-Like Receptors
Intestinal Mucosa
Cell Membrane
Epithelial Cells
Pattern Recognition Receptors
Peptidoglycan
Lipopolysaccharides
Mucous Membrane
Epithelium
Ligands
Bacteria
Cell Line
Membranes
Population

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Commensal-associated molecular patterns induce selective toll-like receptor-trafficking from apical membrane to cytoplasmic compartments in polarized intestinal epithelium. / Cario, Elke; Brown, Dennis; McKee, Mary; Lynch-Devaney, Kathryn; Gerken, Guido; Podolsky, Daniel K.

In: American Journal of Pathology, Vol. 160, No. 1, 2002, p. 165-173.

Research output: Contribution to journalArticle

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AU - Gerken, Guido

AU - Podolsky, Daniel K.

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