TY - JOUR
T1 - Comparable impact of mutational and selective influences in shaping the expressed repertoire of peripheral IgM+/CD5- and IgM+/CD5+ B cells
AU - Dörner, Thomas
AU - Brezinschek, Hans Peter
AU - Foster, Sandra J.
AU - Brezinschek, Ruth I.
AU - Farner, Nancy L.
AU - Lipsky, Peter E.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1998/2
Y1 - 1998/2
N2 - Somatic hypermutation and subsequent selection play a significant role in shaping the peripheral B cell repertoire. This repertoire is composed of CD5+ (5%) and CD5- B cells (95%) which are known to traffic through different lymphoid compartments. Previous studies have shown that V(H) gene usage by CD5+ and CD5- B cells is similar, although mutations are more frequent in the latter. However, the effect of mutation and subsequent selection on the expressed V(H) repertoire of CD5+ and CD5- B cells has not been delineated in detail. This study, therefore, analyzed the mutational pattern of individual IgM+/CD5+ and IgM+/CD5- B cells. In both populations, mutations can occur without heavy chain isotype switching. Despite the differences in mutational frequency, the patterns of mutation and subsequent selection were comparable in CD5+ and CD5- B cells. These results imply that although mutations are more frequent in CD5- B cells, the overall mechanisms governing somatic hypermutation and subsequent positive and negative selection are similar in CD5+ and CD5- B cells.
AB - Somatic hypermutation and subsequent selection play a significant role in shaping the peripheral B cell repertoire. This repertoire is composed of CD5+ (5%) and CD5- B cells (95%) which are known to traffic through different lymphoid compartments. Previous studies have shown that V(H) gene usage by CD5+ and CD5- B cells is similar, although mutations are more frequent in the latter. However, the effect of mutation and subsequent selection on the expressed V(H) repertoire of CD5+ and CD5- B cells has not been delineated in detail. This study, therefore, analyzed the mutational pattern of individual IgM+/CD5+ and IgM+/CD5- B cells. In both populations, mutations can occur without heavy chain isotype switching. Despite the differences in mutational frequency, the patterns of mutation and subsequent selection were comparable in CD5+ and CD5- B cells. These results imply that although mutations are more frequent in CD5- B cells, the overall mechanisms governing somatic hypermutation and subsequent positive and negative selection are similar in CD5+ and CD5- B cells.
KW - Antibody
KW - B lymphocyte
KW - Generation of diversity
KW - Somatic hypermutation
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U2 - 10.1002/(SICI)1521-4141(199802)28:02<657::AID-IMMU657>3.0.CO;2-Z
DO - 10.1002/(SICI)1521-4141(199802)28:02<657::AID-IMMU657>3.0.CO;2-Z
M3 - Article
C2 - 9521076
AN - SCOPUS:0031889119
SN - 0014-2980
VL - 28
SP - 657
EP - 668
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 2
ER -