Comparable impact of mutational and selective influences in shaping the expressed repertoire of peripheral IgM+/CD5- and IgM+/CD5+ B cells

Thomas Dörner, Hans Peter Brezinschek, Sandra J. Foster, Ruth I. Brezinschek, Nancy L. Farner, Peter E. Lipsky

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Somatic hypermutation and subsequent selection play a significant role in shaping the peripheral B cell repertoire. This repertoire is composed of CD5+ (5%) and CD5- B cells (95%) which are known to traffic through different lymphoid compartments. Previous studies have shown that V(H) gene usage by CD5+ and CD5- B cells is similar, although mutations are more frequent in the latter. However, the effect of mutation and subsequent selection on the expressed V(H) repertoire of CD5+ and CD5- B cells has not been delineated in detail. This study, therefore, analyzed the mutational pattern of individual IgM+/CD5+ and IgM+/CD5- B cells. In both populations, mutations can occur without heavy chain isotype switching. Despite the differences in mutational frequency, the patterns of mutation and subsequent selection were comparable in CD5+ and CD5- B cells. These results imply that although mutations are more frequent in CD5- B cells, the overall mechanisms governing somatic hypermutation and subsequent positive and negative selection are similar in CD5+ and CD5- B cells.

Original languageEnglish (US)
Pages (from-to)657-668
Number of pages12
JournalEuropean Journal of Immunology
Volume28
Issue number2
DOIs
StatePublished - Feb 1998

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Immunoglobulin M
B-Lymphocytes
Mutation
Immunoglobulin Class Switching
Mutation Rate
Population
Genes

Keywords

  • Antibody
  • B lymphocyte
  • Generation of diversity
  • Somatic hypermutation

ASJC Scopus subject areas

  • Immunology

Cite this

Comparable impact of mutational and selective influences in shaping the expressed repertoire of peripheral IgM+/CD5- and IgM+/CD5+ B cells. / Dörner, Thomas; Brezinschek, Hans Peter; Foster, Sandra J.; Brezinschek, Ruth I.; Farner, Nancy L.; Lipsky, Peter E.

In: European Journal of Immunology, Vol. 28, No. 2, 02.1998, p. 657-668.

Research output: Contribution to journalArticle

Dörner, Thomas ; Brezinschek, Hans Peter ; Foster, Sandra J. ; Brezinschek, Ruth I. ; Farner, Nancy L. ; Lipsky, Peter E. / Comparable impact of mutational and selective influences in shaping the expressed repertoire of peripheral IgM+/CD5- and IgM+/CD5+ B cells. In: European Journal of Immunology. 1998 ; Vol. 28, No. 2. pp. 657-668.
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