Comparative adhesion of human haemopoietic cell lines to extracellular matrix components, bone marrow stromal and endothelial cultures

Marc L. Turner, Lisa C. Masek, Cheryl L. Hardy, Alistair C. Parker, John W. Sweetenham

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We used flow cytometry to characterize cell adhesion molecule expression of the human haemopoietic cell lines KG1a, K562, HL-60, NALM-6 and CEM. A 51chromium labelling assay was used to study the adhesion of these cell lines to extracellular matrix components and to bone marrow stromal and endothelial cultures. Both adhesion molecule expression and functional binding behaviour varied between cell lines. All five cell lines expressed the integrins α4β1 and α5β1 and all adhered to fibronectin. However, differences in intensity of expression of these integrins failed to correlate with extent of fibronectin adhesion. Inhibition experiments demonstrated that adhesion of KG1a to fibronectin was completely inhibited by divalent cation chelation and partially inhibited by RGDS peptides and chondroitinase ABC, suggesting that both α4β1 and α5β1 as well as CD44 were responsible for this interaction. Adhesion to bone marrow stromal and endothelial layers was superior to that to purified extracellular matrix components and was partially inhibited by divalent cation chelation. RGD peptides and anti-α4 monoclonal antibody also partially inhibited KG1a adhesion to bone marrow endothelium. Discordance between cell adhesion molecule expression and adhesive behaviour suggest that current phenotypic descriptions remain incomplete and reinforce the need for complementary functional binding studies.

Original languageEnglish (US)
Pages (from-to)112-122
Number of pages11
JournalBritish Journal of Haematology
Volume100
Issue number1
DOIs
StatePublished - Feb 6 1998
Externally publishedYes

Fingerprint

Extracellular Matrix
Bone Marrow
Fibronectins
Cell Line
Divalent Cations
Cell Adhesion Molecules
Integrins
arginyl-glycyl-aspartyl-serine
Chondroitin ABC Lyase
Adhesives
Endothelium
Flow Cytometry
Monoclonal Antibodies

Keywords

  • Bone marrow cultures
  • Bone marrow endothelial cultures
  • Cell adhesion
  • Extracellular matrix
  • Haemopoietic progenitor cells

ASJC Scopus subject areas

  • Hematology

Cite this

Comparative adhesion of human haemopoietic cell lines to extracellular matrix components, bone marrow stromal and endothelial cultures. / Turner, Marc L.; Masek, Lisa C.; Hardy, Cheryl L.; Parker, Alistair C.; Sweetenham, John W.

In: British Journal of Haematology, Vol. 100, No. 1, 06.02.1998, p. 112-122.

Research output: Contribution to journalArticle

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