@article{d3289e13f1084e2ab5bbd473eeaf6f6f,
title = "Comparative analysis of viral entry for Asian and African lineages of Zika virus",
abstract = "Zika virus (ZIKV) is an emerging pathogen with global health and economic impacts. ZIKV circulates as two major lineages, Asian or African. The Asian lineage has recently been associated with significant disease in humans. Numerous studies have revealed differences between African and Asian ZIKV strains with respect to cellular infectivity, pathogenesis, and immune activation. Less is known about the mechanism of ZIKV entry and whether viral entry differs between strains. Here, we characterized ZIKV entry with two Asian and two African strains. All viruses exhibited a requirement for clathrin-mediated endocytosis and Rab5a function. Additionally, all ZIKV strains tested were sensitive to pH in the range of 6.5–6.1 and were reliant on endosomal acidification for infection. Finally, we provide direct evidence that ZIKV primarily fuses with late endosomes. These findings contribute new insight into the ZIKV entry process and suggest that divergent ZIKV strains enter cells in a highly conserved manner.",
keywords = "Endosome, Entry, Flavivirus, Internalization, Zika",
author = "Nicholas Rinkenberger and Schoggins, {John W.}",
note = "Funding Information: We thank Jennifer Eitson and Didi Chen for technical assistance, and Katrina Mar for critical manuscript feedback. We acknowledge the assistance of the UT Southwestern Live Cell Imaging Facility, a shared resource of the Harold C. Simmons Cancer Center, supported in part by an NCI Cancer Center Support Grant, CA142543. This work was supported in part by NIH grants AI117922 and by the UT Southwestern Endowed Scholars program (J.W.S.). Additional support for N.R. was obtained from NIH T32 Training Grant AI007520. Funding Information: We thank Jennifer Eitson and Didi Chen for technical assistance, and Katrina Mar for critical manuscript feedback. We acknowledge the assistance of the UT Southwestern Live Cell Imaging Facility, a shared resource of the Harold C. Simmons Cancer Center, supported in part by an NCI Cancer Center Support Grant, CA142543 . This work was supported in part by NIH grants AI117922 and by the UT Southwestern Endowed Scholars program (J.W.S.). Additional support for N.R. was obtained from NIH T32 Training Grant AI007520 . Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = jul,
doi = "10.1016/j.virol.2019.04.008",
language = "English (US)",
volume = "533",
pages = "59--67",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
}