Comparative HPLC-MS n analysis of canine and human meibomian lipidomes: Many similarities, a few differences

Igor A. Butovich, Anna M. Borowiak, J. Corinna Eule

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The aim of this study was to evaluate the lipidome of meibomian gland secretions in canines (cMGS)-a common pet and laboratory animal-and to compare it with that of human MGS (hMGS), to determine whether canines could be used as a valid experimental animal model in studies of the biochemistry and physiology of the human ocular surface in general, and of the Meibomian glands in particular. The MGS of both species were evaluated using HPLC in combination with atmospheric pressure chemical ionization ion trap mass spectrometry. The main lipid classes found in cMGS were very long chain cholesteryl esters, wax esters, (O-acyl)-omega-hydroxy fatty acids (OAHFA), and cholesteryl esters of OAHFA. The lipidomes of cMGS and hMGS were found to be qualitatively similar, which implies similar biosynthetic and biodegradation pathways in canines and humans. However, some quantitative differences between the two were observed.

Original languageEnglish (US)
Article number24
JournalScientific Reports
Volume1
DOIs
StatePublished - 2011

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Canidae
High Pressure Liquid Chromatography
Meibomian Glands
Hydroxy Acids
Cholesterol Esters
Fatty Acids
Ocular Physiological Phenomena
Atmospheric Pressure
Waxes
Pets
Biosynthetic Pathways
Laboratory Animals
Biochemistry
Mass Spectrometry
Esters
Animal Models
Ions
Lipids

ASJC Scopus subject areas

  • General

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Comparative HPLC-MS n analysis of canine and human meibomian lipidomes : Many similarities, a few differences. / Butovich, Igor A.; Borowiak, Anna M.; Eule, J. Corinna.

In: Scientific Reports, Vol. 1, 24, 2011.

Research output: Contribution to journalArticle

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