@article{7cd5b13337354240859db2599bc3a9ad,
title = "Comparative HPLC-MS n analysis of canine and human meibomian lipidomes: Many similarities, a few differences",
abstract = "The aim of this study was to evaluate the lipidome of meibomian gland secretions in canines (cMGS)-a common pet and laboratory animal-and to compare it with that of human MGS (hMGS), to determine whether canines could be used as a valid experimental animal model in studies of the biochemistry and physiology of the human ocular surface in general, and of the Meibomian glands in particular. The MGS of both species were evaluated using HPLC in combination with atmospheric pressure chemical ionization ion trap mass spectrometry. The main lipid classes found in cMGS were very long chain cholesteryl esters, wax esters, (O-acyl)-omega-hydroxy fatty acids (OAHFA), and cholesteryl esters of OAHFA. The lipidomes of cMGS and hMGS were found to be qualitatively similar, which implies similar biosynthetic and biodegradation pathways in canines and humans. However, some quantitative differences between the two were observed.",
author = "Butovich, {Igor A.} and Borowiak, {Anna M.} and Eule, {J. Corinna}",
note = "Funding Information: This work was supported in part by an NIH grant R01EY019480 (to IAB) and an unrestricted grant from Research to Prevent Blindness (New York, NY). Funding Information: Competing financial interests: To comply with the Journal{\textquoteright}s policy on duality of interests, IAB would like to declare that this work was supported solely by his NIH grant R01EY019480. IAB has a sponsored research contract with Alcon Laboratories (USA), and a consulting agreement with Pfizer (USA). The manuscript is based solely on the results obtained within the framework of his current NIH R01 project, and is not related to any other projects in his laboratory. JCE and AMB declare no financial interests. License: This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ How to cite this article: Butovich, I.A., Borowiak, A.M. & Eule, J.C. Comparative HPLC-MSn analysis of canine and human meibomian lipidomes: many similarities, a few differences. Sci. Rep. 1, 24; DOI:10.1038/srep00024 (2011).",
year = "2011",
doi = "10.1038/srep00024",
language = "English (US)",
volume = "1",
journal = "Scientific reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
}