TY - JOUR
T1 - Comparative Proteomic Analysis of Extracellular Vesicles Isolated by Acoustic Trapping or Differential Centrifugation
AU - Rezeli, Melinda
AU - Gidlöf, Olof
AU - Evander, Mikael
AU - Bryl-Górecka, Paulina
AU - Sathanoori, Ramasri
AU - Gilje, Patrik
AU - Pawłowski, Krzysztof
AU - Horvatovich, Péter
AU - Erlinge, David
AU - Marko-Varga, György
AU - Laurell, Thomas
N1 - Funding Information:
This work was supported by grants from the Swedish Foundation for Strategic Research Grant No. SBE13-0049 TOTAL-AMI, Swedish Academy of Pharmaceutical Sciences, Swedish Research Council, Vinnova, Ingabritt & Arne Lundbergs forskningsstiftelse and the Crafoord Foundation.
Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/9/6
Y1 - 2016/9/6
N2 - Extracellular vesicles (ECVs), including microparticles and exosomes, are submicrometer membrane vesicles released by diverse cell types upon activation or stress. Circulating ECVs are potential reservoirs of disease biomarkers, and the complexity of these vesicles is significantly lower compared to their source, blood plasma, which makes ECV-based biomarker studies more promising. Proteomic profiling of ECVs is important not only to discover new diagnostic or prognostic markers but also to understand their roles in biological function. In the current study, we investigated the protein composition of plasma-derived ECVs isolated by acoustic seed trapping. Additionally, the protein composition of ECVs isolated with acoustic trapping was compared to that isolated with a conventional differential centrifugation protocol. Finally, the proteome of ECVs originating from ST-elevation myocardial infarction patients was compared with that of healthy controls using label-free LC-MS quantification. The acoustic trapping platform allows rapid and automated preparation of ECVs from small sample volumes, which are therefore well-suited for biobank repositories. We found that the protein composition of trapped ECVs is very similar to that isolated by the conventional differential centrifugation method.
AB - Extracellular vesicles (ECVs), including microparticles and exosomes, are submicrometer membrane vesicles released by diverse cell types upon activation or stress. Circulating ECVs are potential reservoirs of disease biomarkers, and the complexity of these vesicles is significantly lower compared to their source, blood plasma, which makes ECV-based biomarker studies more promising. Proteomic profiling of ECVs is important not only to discover new diagnostic or prognostic markers but also to understand their roles in biological function. In the current study, we investigated the protein composition of plasma-derived ECVs isolated by acoustic seed trapping. Additionally, the protein composition of ECVs isolated with acoustic trapping was compared to that isolated with a conventional differential centrifugation protocol. Finally, the proteome of ECVs originating from ST-elevation myocardial infarction patients was compared with that of healthy controls using label-free LC-MS quantification. The acoustic trapping platform allows rapid and automated preparation of ECVs from small sample volumes, which are therefore well-suited for biobank repositories. We found that the protein composition of trapped ECVs is very similar to that isolated by the conventional differential centrifugation method.
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U2 - 10.1021/acs.analchem.6b01694
DO - 10.1021/acs.analchem.6b01694
M3 - Article
C2 - 27487081
AN - SCOPUS:84985896357
SN - 0003-2700
VL - 88
SP - 8577
EP - 8586
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 17
ER -