Comparative Proteomic Analysis of Extracellular Vesicles Isolated by Acoustic Trapping or Differential Centrifugation

Melinda Rezeli, Olof Gidlöf, Mikael Evander, Paulina Bryl-Górecka, Ramasri Sathanoori, Patrik Gilje, Krzysztof Pawłowski, Péter Horvatovich, David Erlinge, György Marko-Varga, Thomas Laurell

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Extracellular vesicles (ECVs), including microparticles and exosomes, are submicrometer membrane vesicles released by diverse cell types upon activation or stress. Circulating ECVs are potential reservoirs of disease biomarkers, and the complexity of these vesicles is significantly lower compared to their source, blood plasma, which makes ECV-based biomarker studies more promising. Proteomic profiling of ECVs is important not only to discover new diagnostic or prognostic markers but also to understand their roles in biological function. In the current study, we investigated the protein composition of plasma-derived ECVs isolated by acoustic seed trapping. Additionally, the protein composition of ECVs isolated with acoustic trapping was compared to that isolated with a conventional differential centrifugation protocol. Finally, the proteome of ECVs originating from ST-elevation myocardial infarction patients was compared with that of healthy controls using label-free LC-MS quantification. The acoustic trapping platform allows rapid and automated preparation of ECVs from small sample volumes, which are therefore well-suited for biobank repositories. We found that the protein composition of trapped ECVs is very similar to that isolated by the conventional differential centrifugation method.

Original languageEnglish (US)
Pages (from-to)8577-8586
Number of pages10
JournalAnalytical Chemistry
Volume88
Issue number17
DOIs
StatePublished - Sep 6 2016
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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