Abstract
Cytogenetic and fluorescence in situ hybridization (FISH) studies permit analyses of structural and numerical chromosome abnormalities. We compared modal chromosome count in cancer cells by FISH and classical cytogenetics (CC). Our cytogentic studies for chromosomes 3 and 17 on 134 human solid tumor cultures {57 small cell lung cancer (SCLC) and 77 non small cell lung cancers (NSCLC)}, indicated a high incidence of numerical changes in these tumors. Using centromeric probes for chromosomes 3 and 17 we compared 15 tumor and 3 lymphoblastoid cultures, both by FISH (> 100 interphase nuclei) and by CC using trypsin-Giemsa G banding (> 20 metaphases). Although some intra-tumor heterogeneity was seen by FISH, we observed a high degree of concordancy (78%) in modal count by the two techniques.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1351-1356 |
| Number of pages | 6 |
| Journal | Anticancer Research |
| Volume | 18 |
| Issue number | 3 A |
| State | Published - May 1998 |
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Keywords
- Breast
- Classical cytogenetics
- Fluorescence in situ hybridization
- Lung
- Metaphase
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Comparison between fluorescence in situ hybridization and classical cytogenetics in human tumors. / Virmani, Arvind K.; Tonk, Vijay S.; Gazdar, Adi F.
In: Anticancer Research, Vol. 18, No. 3 A, 05.1998, p. 1351-1356.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison between fluorescence in situ hybridization and classical cytogenetics in human tumors
AU - Virmani, Arvind K.
AU - Tonk, Vijay S.
AU - Gazdar, Adi F.
PY - 1998/5
Y1 - 1998/5
N2 - Cytogenetic and fluorescence in situ hybridization (FISH) studies permit analyses of structural and numerical chromosome abnormalities. We compared modal chromosome count in cancer cells by FISH and classical cytogenetics (CC). Our cytogentic studies for chromosomes 3 and 17 on 134 human solid tumor cultures {57 small cell lung cancer (SCLC) and 77 non small cell lung cancers (NSCLC)}, indicated a high incidence of numerical changes in these tumors. Using centromeric probes for chromosomes 3 and 17 we compared 15 tumor and 3 lymphoblastoid cultures, both by FISH (> 100 interphase nuclei) and by CC using trypsin-Giemsa G banding (> 20 metaphases). Although some intra-tumor heterogeneity was seen by FISH, we observed a high degree of concordancy (78%) in modal count by the two techniques.
AB - Cytogenetic and fluorescence in situ hybridization (FISH) studies permit analyses of structural and numerical chromosome abnormalities. We compared modal chromosome count in cancer cells by FISH and classical cytogenetics (CC). Our cytogentic studies for chromosomes 3 and 17 on 134 human solid tumor cultures {57 small cell lung cancer (SCLC) and 77 non small cell lung cancers (NSCLC)}, indicated a high incidence of numerical changes in these tumors. Using centromeric probes for chromosomes 3 and 17 we compared 15 tumor and 3 lymphoblastoid cultures, both by FISH (> 100 interphase nuclei) and by CC using trypsin-Giemsa G banding (> 20 metaphases). Although some intra-tumor heterogeneity was seen by FISH, we observed a high degree of concordancy (78%) in modal count by the two techniques.
KW - Breast
KW - Classical cytogenetics
KW - Fluorescence in situ hybridization
KW - Lung
KW - Metaphase
UR - http://www.scopus.com/inward/record.url?scp=0031854670&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031854670&partnerID=8YFLogxK
M3 - Article
C2 - 9673339
AN - SCOPUS:0031854670
VL - 18
SP - 1351
EP - 1356
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 3 A
ER -