Comparison of [3,4-13C2]glucose to [6,6- 2H2]glucose as a tracer for glucose turnover by nuclear magnetic resonance

Eunsook S. Jin, John G. Jones, Shawn C. Burgess, Matthew E. Merritt, A. Dean Sherry, Craig R. Malloy

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

A recently introduced tracer, [3,4-13C2]glucose, was compared to the widely used tracer, [6,6-2H2]glucose, for measurement of whole-body glucose turnover. The rate of glucose production (GP) was measured in rats after primed infusions of [3,4-13C 2]glucose, [6,6-2H2]glucose, or both tracers simultaneously followed by a constant infusion of tracer(s) over 90 min. Blood glucose was purified and converted into monoacetone glucose for analysis by 13C NMR (for [3,4-13C2]glucose) or 1H and 2H NMR (for [6,6-2H2]glucose) . The values of GP measured during infusion of each single tracer were not significantly different. In rats infused with both tracers simultaneously, GP was identical as reported by each tracer, 42 ± 4 μmol/kg/min. Since 2H and 13C enrichment in glucose is typically much less than 2% for in vivo studies, [3,4-13C2]glucose does not interfere with measurements of 13C or 2H enrichment patterns and therefore is valuable when multiple metabolic pathways are being evaluated simultaneously.

Original languageEnglish (US)
Pages (from-to)1479-1483
Number of pages5
JournalMagnetic resonance in medicine
Volume53
Issue number6
DOIs
StatePublished - Jun 2005

Keywords

  • C
  • Glucose turnover
  • H
  • NMR
  • Stable isotope tracers

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'Comparison of [3,4-<sup>13</sup>C<sub>2</sub>]glucose to [6,6- <sup>2</sup>H<sub>2</sub>]glucose as a tracer for glucose turnover by nuclear magnetic resonance'. Together they form a unique fingerprint.

  • Cite this