Comparison of 4th-generation HIV antigen/ antibody combination assay with 3rd-generation HIV antibody assays for the occurrence of false-positive and false-negative results

Alagarraju Muthukumar, Adnan Alatoom, Susan Burns, Jerry Ashmore, Anne Kim, Brian Emerson, Edward Bannister, M. Qasim Ansari

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective: To assess the false-positive and false-negative rates of a 4th-generation human immunodeficiency virus (HIV) assay, the Abbott ARCHITECT, vs 2 HIV 3rd-generation assays, the Siemens Centaur and the Ortho-Clinical Diagnostics Vitros. Methods: We examined 123 patient specimens. In the first phase of the study, we compared 99 specimens that had a positive screening result via the 3rd-generation Vitros assay (10 positive, 82 negative, and 7 indeterminate via confirmatory immunofluorescent assay [IFA]/ Western blot [WB] testing). In the second phase, we assessed 24 HIV-1 RNA-positive (positive result via the nuclear acid amplification test [NAAT] and negative/indeterminate results via the WB test) specimens harboring acute HIV infection. Results: The 4th-generation ARCHITECT assay yielded fewer falsepositive results (n = 2) than the 3rd-generation Centaur (n = 9; P = .02) and Vitros (n = 82; P

Original languageEnglish (US)
Pages (from-to)84-89
Number of pages6
JournalLaboratory Medicine
Volume46
Issue number2
DOIs
StatePublished - May 1 2015

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Viruses
Assays
Western Blotting
HIV
Antigens
HIV-2
Antibodies
Virus Diseases
HIV-1
RNA
Acids
Amplification
Screening
Testing

Keywords

  • 3rd-generation HIV assays
  • 4th-generation HIV testing
  • False-negative results
  • False-positive results
  • HIV
  • Western blot

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Comparison of 4th-generation HIV antigen/ antibody combination assay with 3rd-generation HIV antibody assays for the occurrence of false-positive and false-negative results. / Muthukumar, Alagarraju; Alatoom, Adnan; Burns, Susan; Ashmore, Jerry; Kim, Anne; Emerson, Brian; Bannister, Edward; Ansari, M. Qasim.

In: Laboratory Medicine, Vol. 46, No. 2, 01.05.2015, p. 84-89.

Research output: Contribution to journalArticle

Muthukumar, Alagarraju ; Alatoom, Adnan ; Burns, Susan ; Ashmore, Jerry ; Kim, Anne ; Emerson, Brian ; Bannister, Edward ; Ansari, M. Qasim. / Comparison of 4th-generation HIV antigen/ antibody combination assay with 3rd-generation HIV antibody assays for the occurrence of false-positive and false-negative results. In: Laboratory Medicine. 2015 ; Vol. 46, No. 2. pp. 84-89.
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