TY - GEN
T1 - Comparison of contractile force generated by isolated and paired fibroblasts in vitro
AU - Mariappan, M. R.
AU - Petroll, Walter M
AU - Cavanagh, Harrison D
AU - Jester, J. V.
PY - 1999/12/1
Y1 - 1999/12/1
N2 - Wound contraction is mediated by inter-connected fibroblasts that generate contractile forces on the extracellular matrix. To begin examining the contractile nature of inter-connected fibroblasts, we used an in vitro biophysical system to measure force generated by isolated (IF) and paired fibroblasts (PF). The biophysical system consists of collagen gel with fiduciary micro-beads that serve as reference points in the matrix. Fibroblasts cultured in DMEM with serum were trypsinized and plated on top of the collagen gel in serum free conditions. Images of IF or PF are digitized every minute for 30 minutes before and after application of lyso-phosphatidic acid (LPA), which induces fibroblast contraction. Contracting fibroblasts deform the collagen matrix resulting in displacement of fiduciary beads. Time lapse video microscopic analysis was used to track bead displacement every minute. Force generated by IF or PF were measured from bead displacement and matrix stiffness. Our preliminary results indicate that PFs generate relatively higher (p<0.05, two tailed t-test) contractile forces (mean force = (2.8±1.1)×10-8 N, mean±SD, n = 6), when compared to IFs ((1.5±0.9)×10-8 N, n = 6).
AB - Wound contraction is mediated by inter-connected fibroblasts that generate contractile forces on the extracellular matrix. To begin examining the contractile nature of inter-connected fibroblasts, we used an in vitro biophysical system to measure force generated by isolated (IF) and paired fibroblasts (PF). The biophysical system consists of collagen gel with fiduciary micro-beads that serve as reference points in the matrix. Fibroblasts cultured in DMEM with serum were trypsinized and plated on top of the collagen gel in serum free conditions. Images of IF or PF are digitized every minute for 30 minutes before and after application of lyso-phosphatidic acid (LPA), which induces fibroblast contraction. Contracting fibroblasts deform the collagen matrix resulting in displacement of fiduciary beads. Time lapse video microscopic analysis was used to track bead displacement every minute. Force generated by IF or PF were measured from bead displacement and matrix stiffness. Our preliminary results indicate that PFs generate relatively higher (p<0.05, two tailed t-test) contractile forces (mean force = (2.8±1.1)×10-8 N, mean±SD, n = 6), when compared to IFs ((1.5±0.9)×10-8 N, n = 6).
UR - http://www.scopus.com/inward/record.url?scp=0033345250&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033345250&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:0033345250
SN - 0780356756
T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
BT - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PB - IEEE
T2 - Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
Y2 - 13 October 1999 through 16 October 1999
ER -