Comparison of contractile force generated by isolated and paired fibroblasts in vitro

M. R. Mariappan, Walter M Petroll, Harrison D Cavanagh, J. V. Jester

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Wound contraction is mediated by inter-connected fibroblasts that generate contractile forces on the extracellular matrix. To begin examining the contractile nature of inter-connected fibroblasts, we used an in vitro biophysical system to measure force generated by isolated (IF) and paired fibroblasts (PF). The biophysical system consists of collagen gel with fiduciary micro-beads that serve as reference points in the matrix. Fibroblasts cultured in DMEM with serum were trypsinized and plated on top of the collagen gel in serum free conditions. Images of IF or PF are digitized every minute for 30 minutes before and after application of lyso-phosphatidic acid (LPA), which induces fibroblast contraction. Contracting fibroblasts deform the collagen matrix resulting in displacement of fiduciary beads. Time lapse video microscopic analysis was used to track bead displacement every minute. Force generated by IF or PF were measured from bead displacement and matrix stiffness. Our preliminary results indicate that PFs generate relatively higher (p<0.05, two tailed t-test) contractile forces (mean force = (2.8±1.1)×10-8 N, mean±SD, n = 6), when compared to IFs ((1.5±0.9)×10-8 N, n = 6).

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Place of PublicationPiscataway, NJ, United States
PublisherIEEE
Pages99
Number of pages1
Volume1
ISBN (Print)0780356756
Publication statusPublished - 1999
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

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ASJC Scopus subject areas

  • Bioengineering

Cite this

Mariappan, M. R., Petroll, W. M., Cavanagh, H. D., & Jester, J. V. (1999). Comparison of contractile force generated by isolated and paired fibroblasts in vitro. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 1, pp. 99). Piscataway, NJ, United States: IEEE.