Comparison of four cytokine regimens for mobilization of peripheral blood stem cells: IL-3 alone and combined with GM-CSF or G-CSF

C. S. Rosenfeld, B. Bolwell, A. LeFever, R. Taylor, A. List, J. Fay, R. Collins, F. Andrews, P. Pallansch, M. W. Schuster, D. Resta, D. Levitt, J. Nemunaitis

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Improved methods for mobilization may reduce the number of aphereses required to collect adequate numbers of peripheral blood stem cells (PBSC) and hasten engraftment following high-dose therapy. Mobilization with cytokines alone enables engraftment after myeloablative therapy. The optimal cytokine regimen for mobilization has not been established. The study evaluated the effects of four interleukin-3-containing cytokine regimens administered during steady state hematopoiesis on PBSC mobilization in 30 patients with breast cancer or lymphoid malignancies. These regimens included IL-3 alone (Arm 1), sequential IL-3 → G-CSF (Arm 2), sequential IL-3 → GM-CSF (Arm 3) and combined IL-3 + G-CSF (Arm 4). Consecutive days of apheresis were performed until a target of 4-6 x 108 mononuclear cells/kg were collected. All patients received intravenous GM-CSF after PBSC infusion. Median days to an ANC ≥ 500/μl in Arm 3 (22 days) was significantly slower than for patients in Arm 2 (13 days) but not significantly different from patients in Arm 1 or Arm 4. There was no significant difference in platelet engraftment or days of hospitalization between the study arms. Addition of GM-CSF to IL-3-containing mobilization regimens results in collection of PBSC that lead to delayed engraftment. Further development of Arms 1, 2, and 4 appear warranted.

Original languageEnglish (US)
Pages (from-to)179-183
Number of pages5
JournalBone Marrow Transplantation
Volume17
Issue number2
StatePublished - Feb 1 1996

Keywords

  • Autologous bone marrow transplantation
  • Breast neoplasms
  • Granulocyte-macrophage colony-stimulating factor
  • Interleukin-3
  • Lymphoma
  • Transplantation

ASJC Scopus subject areas

  • Hematology
  • Transplantation

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