Comparison of identification systems for Staphylococcus epidermidis and other coagulase-negative Staphylococcus species

Trish M. Perl, Paul R. Rhomberg, Martha J. Bale, Peter C. Fuchs, Ronald N. Jones, Franklin P. Koontz, Michael A. Pfaller

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Three commercially available systems (API Staph-Trac, API 20GP, and Vitek GPI), used to identify coagulase-negative staphylococci, were evaluated against 277 bloodstream isolates, including 94 isolates of Staphylococcus epidermidis and 183 isolates of other coagulase-negative Staphylococcus species. The conventional method of Kloos and Schleifer served as the reference method. Controls included 14 ATCC type culture strains of coagulase-negative staphylococci. The API Staph-Trac system showed the highest rate of agreement with reference method, correctly identifying 73% of the isolates. The Vitek GPI System had an overall rate of agreement of 67% and the API 20GP system correctly identified 61%. The API Staph-Trac system correctly identified 94% of the isolates of S. epidermidis compared with 64% by both Vitek GPI and API 20GP. The most common error for both Vitek GPI and API 20GP systems was the failure to identify organisms contained within the database of the systems. Because none of the tested commercial identification systems identified "non-epidermidis" coagulase-negative Staphylococcus species with a high degree of accuracy, the systems need to be markedly improved or new systems developed.

Original languageEnglish (US)
Pages (from-to)151-155
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume18
Issue number3
DOIs
StatePublished - Mar 1994

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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