Comparison of Optical and Power Doppler Ultrasound Imaging for Non-Invasive Evaluation of Arsenic Trioxide as a Vascular Disrupting Agent in Tumors

Mustafa K. Alhasan, Li Liu, Matthew A. Lewis, Jennifer Magnusson, Ralph P. Mason

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Small animal imaging provides diverse methods for evaluating tumor growth and acute response to therapy. This study compared the utility of non-invasive optical and ultrasound imaging to monitor growth of three diverse human tumor xenografts (brain U87-luc-mCherry, mammary MCF7-luc-mCherry, and prostate PC3-luc) growing in nude mice. Bioluminescence imaging (BLI), fluorescence imaging (FLI), and Power Doppler ultrasound (PD US) were then applied to examine acute vascular disruption following administration of arsenic trioxide (ATO). During initial tumor growth, strong correlations were found between manual caliper measured tumor volume and FLI intensity, BLI intensity following luciferin injection, and traditional B-mode US. Administration of ATO to established U87 tumors caused significant vascular shutdown within 2 hrs at all doses in the range 5 to 10 mg/kg in a dose dependant manner, as revealed by depressed bioluminescent light emission. At lower doses substantial recovery was seen within 4 hrs. At 8 mg/kg there was >85% reduction in tumor vascular perfusion, which remained depressed after 6 hrs, but showed some recovery after 24 hrs. Similar response was observed in MCF7 and PC3 tumors. Dynamic BLI and PD US each showed similar duration and percent reductions in tumor blood flow, but FLI showed no significant changes during the first 24 hrs. The results provide further evidence for comparable utility of optical and ultrasound imaging for monitoring tumor growth, More specifically, they confirm the utility of BLI and ultrasound imaging as facile assays of the vascular disruption in solid tumors based on ATO as a model agent.

Original languageEnglish (US)
Article numbere46106
JournalPLoS One
Volume7
Issue number9
DOIs
StatePublished - Sep 28 2012

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Doppler Ultrasonography
arsenic
blood vessels
ultrasonography
Blood Vessels
Tumors
Ultrasonics
Optical Imaging
Imaging techniques
neoplasms
image analysis
Bioluminescence
bioluminescence
Neoplasms
Ultrasonography
Growth
fluorescence
Fluorescence
dosage
luciferin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Comparison of Optical and Power Doppler Ultrasound Imaging for Non-Invasive Evaluation of Arsenic Trioxide as a Vascular Disrupting Agent in Tumors. / Alhasan, Mustafa K.; Liu, Li; Lewis, Matthew A.; Magnusson, Jennifer; Mason, Ralph P.

In: PLoS One, Vol. 7, No. 9, e46106, 28.09.2012.

Research output: Contribution to journalArticle

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