Comparison of p53 gene mutations in paired primary and metastatic gastric tumor tissues.

J. H. Kim, J. J. Choi, S. H. Noh, J. K. Roh, J. S. Min, J. K. Youn, N. C. Yoo, H. Y. Lim, D. P. Carbone, A. F. Gazdar

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Our previous study revealed that mutations of the p53 gene were detected by cDNA sequencing in one of four (25%) primary gastric tumors and in five of six (83%) gastric cancer cell lines. It was of interest that all five cell lines established from metastatic lesions had p53 gene mutations, while the single cell line established from a primary tumor lacked an abnormality. Thus, the current study was initiated to determine the frequency of p53 mutations in 10 pairs of samples from primary gastric carcinomas and their lymph node metastases, in addition to morphologically normal gastric mucosa. In addition, we correlated the findings with other relevant molecular markers including the metastasis associated nm23-H1 gene and loss of heterozygosity (LOH) using multiple polymorphic markers for chromosome 17p and sequencing the entire open reading frame (ORF) of the p53 gene. Five of ten (50%) patients were constitutionally heterozygous for one or more 17p and/or p53 probes (pYNZ 22, BamHI RFLP; pMct35.1, Mspl RFLP; php53cl, Bg/II RFLP), while none had LOH at the 17p and/or p53. A Bg/II RFLP for analysis of possible nm23-H1 somatic allelic deletion revealed no LOH out of four informative cases. One paired sample demonstrated the substitution of valine for isoleucine at codon 41 (GTT to ATT) in both primary gastric tumor and metastasis. Another metastatic sample demonstrated the substitution of proline for threonine at codon 278 (CCT to C/ACT) in addition to a non-mutated codon, while only the wild-type p53 sequence was present in the paired primary gastric tumor tissue.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish (US)
Pages (from-to)187-191
Number of pages5
JournalJournal of Korean Medical Science
Volume8
Issue number3
StatePublished - Jun 1993

Fingerprint

p53 Genes
Restriction Fragment Length Polymorphisms
Loss of Heterozygosity
Stomach
Codon
Mutation
Neoplasm Metastasis
Cell Line
Neoplasms
Isoleucine
Valine
Mutation Rate
Threonine
Gastric Mucosa
Genetic Markers
Proline
Open Reading Frames
Stomach Neoplasms
Complementary DNA
Lymph Nodes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Kim, J. H., Choi, J. J., Noh, S. H., Roh, J. K., Min, J. S., Youn, J. K., ... Gazdar, A. F. (1993). Comparison of p53 gene mutations in paired primary and metastatic gastric tumor tissues. Journal of Korean Medical Science, 8(3), 187-191.

Comparison of p53 gene mutations in paired primary and metastatic gastric tumor tissues. / Kim, J. H.; Choi, J. J.; Noh, S. H.; Roh, J. K.; Min, J. S.; Youn, J. K.; Yoo, N. C.; Lim, H. Y.; Carbone, D. P.; Gazdar, A. F.

In: Journal of Korean Medical Science, Vol. 8, No. 3, 06.1993, p. 187-191.

Research output: Contribution to journalArticle

Kim, JH, Choi, JJ, Noh, SH, Roh, JK, Min, JS, Youn, JK, Yoo, NC, Lim, HY, Carbone, DP & Gazdar, AF 1993, 'Comparison of p53 gene mutations in paired primary and metastatic gastric tumor tissues.', Journal of Korean Medical Science, vol. 8, no. 3, pp. 187-191.
Kim JH, Choi JJ, Noh SH, Roh JK, Min JS, Youn JK et al. Comparison of p53 gene mutations in paired primary and metastatic gastric tumor tissues. Journal of Korean Medical Science. 1993 Jun;8(3):187-191.
Kim, J. H. ; Choi, J. J. ; Noh, S. H. ; Roh, J. K. ; Min, J. S. ; Youn, J. K. ; Yoo, N. C. ; Lim, H. Y. ; Carbone, D. P. ; Gazdar, A. F. / Comparison of p53 gene mutations in paired primary and metastatic gastric tumor tissues. In: Journal of Korean Medical Science. 1993 ; Vol. 8, No. 3. pp. 187-191.
@article{068d4ed4d7b745b2b2dfed1d47dbbba2,
title = "Comparison of p53 gene mutations in paired primary and metastatic gastric tumor tissues.",
abstract = "Our previous study revealed that mutations of the p53 gene were detected by cDNA sequencing in one of four (25{\%}) primary gastric tumors and in five of six (83{\%}) gastric cancer cell lines. It was of interest that all five cell lines established from metastatic lesions had p53 gene mutations, while the single cell line established from a primary tumor lacked an abnormality. Thus, the current study was initiated to determine the frequency of p53 mutations in 10 pairs of samples from primary gastric carcinomas and their lymph node metastases, in addition to morphologically normal gastric mucosa. In addition, we correlated the findings with other relevant molecular markers including the metastasis associated nm23-H1 gene and loss of heterozygosity (LOH) using multiple polymorphic markers for chromosome 17p and sequencing the entire open reading frame (ORF) of the p53 gene. Five of ten (50{\%}) patients were constitutionally heterozygous for one or more 17p and/or p53 probes (pYNZ 22, BamHI RFLP; pMct35.1, Mspl RFLP; php53cl, Bg/II RFLP), while none had LOH at the 17p and/or p53. A Bg/II RFLP for analysis of possible nm23-H1 somatic allelic deletion revealed no LOH out of four informative cases. One paired sample demonstrated the substitution of valine for isoleucine at codon 41 (GTT to ATT) in both primary gastric tumor and metastasis. Another metastatic sample demonstrated the substitution of proline for threonine at codon 278 (CCT to C/ACT) in addition to a non-mutated codon, while only the wild-type p53 sequence was present in the paired primary gastric tumor tissue.(ABSTRACT TRUNCATED AT 250 WORDS)",
author = "Kim, {J. H.} and Choi, {J. J.} and Noh, {S. H.} and Roh, {J. K.} and Min, {J. S.} and Youn, {J. K.} and Yoo, {N. C.} and Lim, {H. Y.} and Carbone, {D. P.} and Gazdar, {A. F.}",
year = "1993",
month = "6",
language = "English (US)",
volume = "8",
pages = "187--191",
journal = "Journal of Korean Medical Science",
issn = "1011-8934",
publisher = "Korean Academy of Medical Science",
number = "3",

}

TY - JOUR

T1 - Comparison of p53 gene mutations in paired primary and metastatic gastric tumor tissues.

AU - Kim, J. H.

AU - Choi, J. J.

AU - Noh, S. H.

AU - Roh, J. K.

AU - Min, J. S.

AU - Youn, J. K.

AU - Yoo, N. C.

AU - Lim, H. Y.

AU - Carbone, D. P.

AU - Gazdar, A. F.

PY - 1993/6

Y1 - 1993/6

N2 - Our previous study revealed that mutations of the p53 gene were detected by cDNA sequencing in one of four (25%) primary gastric tumors and in five of six (83%) gastric cancer cell lines. It was of interest that all five cell lines established from metastatic lesions had p53 gene mutations, while the single cell line established from a primary tumor lacked an abnormality. Thus, the current study was initiated to determine the frequency of p53 mutations in 10 pairs of samples from primary gastric carcinomas and their lymph node metastases, in addition to morphologically normal gastric mucosa. In addition, we correlated the findings with other relevant molecular markers including the metastasis associated nm23-H1 gene and loss of heterozygosity (LOH) using multiple polymorphic markers for chromosome 17p and sequencing the entire open reading frame (ORF) of the p53 gene. Five of ten (50%) patients were constitutionally heterozygous for one or more 17p and/or p53 probes (pYNZ 22, BamHI RFLP; pMct35.1, Mspl RFLP; php53cl, Bg/II RFLP), while none had LOH at the 17p and/or p53. A Bg/II RFLP for analysis of possible nm23-H1 somatic allelic deletion revealed no LOH out of four informative cases. One paired sample demonstrated the substitution of valine for isoleucine at codon 41 (GTT to ATT) in both primary gastric tumor and metastasis. Another metastatic sample demonstrated the substitution of proline for threonine at codon 278 (CCT to C/ACT) in addition to a non-mutated codon, while only the wild-type p53 sequence was present in the paired primary gastric tumor tissue.(ABSTRACT TRUNCATED AT 250 WORDS)

AB - Our previous study revealed that mutations of the p53 gene were detected by cDNA sequencing in one of four (25%) primary gastric tumors and in five of six (83%) gastric cancer cell lines. It was of interest that all five cell lines established from metastatic lesions had p53 gene mutations, while the single cell line established from a primary tumor lacked an abnormality. Thus, the current study was initiated to determine the frequency of p53 mutations in 10 pairs of samples from primary gastric carcinomas and their lymph node metastases, in addition to morphologically normal gastric mucosa. In addition, we correlated the findings with other relevant molecular markers including the metastasis associated nm23-H1 gene and loss of heterozygosity (LOH) using multiple polymorphic markers for chromosome 17p and sequencing the entire open reading frame (ORF) of the p53 gene. Five of ten (50%) patients were constitutionally heterozygous for one or more 17p and/or p53 probes (pYNZ 22, BamHI RFLP; pMct35.1, Mspl RFLP; php53cl, Bg/II RFLP), while none had LOH at the 17p and/or p53. A Bg/II RFLP for analysis of possible nm23-H1 somatic allelic deletion revealed no LOH out of four informative cases. One paired sample demonstrated the substitution of valine for isoleucine at codon 41 (GTT to ATT) in both primary gastric tumor and metastasis. Another metastatic sample demonstrated the substitution of proline for threonine at codon 278 (CCT to C/ACT) in addition to a non-mutated codon, while only the wild-type p53 sequence was present in the paired primary gastric tumor tissue.(ABSTRACT TRUNCATED AT 250 WORDS)

UR - http://www.scopus.com/inward/record.url?scp=0027605527&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027605527&partnerID=8YFLogxK

M3 - Article

VL - 8

SP - 187

EP - 191

JO - Journal of Korean Medical Science

JF - Journal of Korean Medical Science

SN - 1011-8934

IS - 3

ER -