Comparison of saliva PCR assay versus rapid culture for detection of congenital cytomegalovirus infection

Swetha G. Pinninti; Shannon A. Ross; Masako Shimamura; Zdenek Novak; April L. Palmer; Amina Ahmed; Robert W. Tolan; David I. Bernstein; Marian G. Michaels; Pablo J. Sánchez; Karen B. Fowler; Suresh B. Boppana

doi:10.1097/INF.0000000000000609

Comparison of saliva PCR assay versus rapid culture for detection of congenital cytomegalovirus infection

Swetha G. Pinninti, Shannon A. Ross, Masako Shimamura, Zdenek Novak, April L. Palmer, Amina Ahmed, Robert W. Tolan, David I. Bernstein, Marian G. Michaels, Pablo J. Sánchez, Karen B. Fowler, Suresh B. Boppana

Pediatrics

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

As part of the CMV and Hearing Multicenter Screening (CHIMES) study, 72,239 newborns were screened for cytomegalovirus by rapid culture and real-time PCR of saliva samples. Of the 266 infants with congenital cytomegalovirus infection, discordance between rapid culture and PCR was observed in 14 children, and 13 were identified only by PCR, demonstrating the superiority of the PCR assay.

Original language	English (US)
Pages (from-to)	536-537
Number of pages	2
Journal	Pediatric Infectious Disease Journal
Volume	34
Issue number	5
DOIs	https://doi.org/10.1097/INF.0000000000000609
State	Published - May 21 2015

Keywords

congenital CMV
hearing loss
late-onset
saliva

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health
Microbiology (medical)
Infectious Diseases

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10.1097/INF.0000000000000609

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Pinninti, S. G., Ross, S. A., Shimamura, M., Novak, Z., Palmer, A. L., Ahmed, A., Tolan, R. W., Bernstein, D. I., Michaels, M. G., Sánchez, P. J., Fowler, K. B., & Boppana, S. B. (2015). Comparison of saliva PCR assay versus rapid culture for detection of congenital cytomegalovirus infection. Pediatric Infectious Disease Journal, 34(5), 536-537. https://doi.org/10.1097/INF.0000000000000609

Pinninti, SG, Ross, SA, Shimamura, M, Novak, Z, Palmer, AL, Ahmed, A, Tolan, RW, Bernstein, DI, Michaels, MG, Sánchez, PJ, Fowler, KB & Boppana, SB 2015, 'Comparison of saliva PCR assay versus rapid culture for detection of congenital cytomegalovirus infection', Pediatric Infectious Disease Journal, vol. 34, no. 5, pp. 536-537. https://doi.org/10.1097/INF.0000000000000609

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abstract = "As part of the CMV and Hearing Multicenter Screening (CHIMES) study, 72,239 newborns were screened for cytomegalovirus by rapid culture and real-time PCR of saliva samples. Of the 266 infants with congenital cytomegalovirus infection, discordance between rapid culture and PCR was observed in 14 children, and 13 were identified only by PCR, demonstrating the superiority of the PCR assay.",

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AU - Pinninti, Swetha G.

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AU - Shimamura, Masako

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AU - Palmer, April L.

AU - Ahmed, Amina

AU - Tolan, Robert W.

AU - Bernstein, David I.

AU - Michaels, Marian G.

AU - Sánchez, Pablo J.

AU - Fowler, Karen B.

AU - Boppana, Suresh B.

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AB - As part of the CMV and Hearing Multicenter Screening (CHIMES) study, 72,239 newborns were screened for cytomegalovirus by rapid culture and real-time PCR of saliva samples. Of the 266 infants with congenital cytomegalovirus infection, discordance between rapid culture and PCR was observed in 14 children, and 13 were identified only by PCR, demonstrating the superiority of the PCR assay.

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KW - hearing loss

KW - late-onset

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Comparison of saliva PCR assay versus rapid culture for detection of congenital cytomegalovirus infection

Abstract

Keywords

ASJC Scopus subject areas

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