Comparison of silicon-coated nylon suture to plain nylon suture in the rat middle cerebral artery occlusion model

Norihito Shimamura, Gerald Matchett, Tamiji Tsubokawa, Hiroki Ohkuma, John Zhang

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

A variety of intraluminal sutures have been used in the middle cerebral artery occlusion model (MCAO) of focal ischemia. In the present study we tested commercially available silicon-coated nylon suture in the MCAO model and compared the results to traditional monofilament nylon suture occlusion. Twelve Sprague-Dawley male rats were randomly divided two groups, MCAO with 4-0 nylon suture (Group N, n = 6) and MCAO with silicone-coated 4-0 nylon suture (Group S, n = 6). Rats were sacrificed 24 h after reperfusion. Assessment included mortality rates, neurological evaluation, and infarct volume. One rat died in each group from subarachanoid hemorrhage. Neurological evaluation demonstrated that Group S tended to have worse neurological outcomes than Group N, although this difference was not statistically significant. On TTC stain Group S had significantly larger infarct volumes than Group N. We conclude that the commercially available silicone-coated occlusion suture provides better occlusion of the middle cerebral artery than the traditional uncoated nylon suture. Classification: Disease-related neuroscience (Section 6).

Original languageEnglish (US)
Pages (from-to)161-165
Number of pages5
JournalJournal of Neuroscience Methods
Volume156
Issue number1-2
DOIs
StatePublished - Sep 30 2006

Fingerprint

Middle Cerebral Artery Infarction
Nylons
Silicon
Sutures
Silicones
Neurosciences
Reperfusion
Sprague Dawley Rats
Coloring Agents
Ischemia
Hemorrhage
Mortality

Keywords

  • Cerebral ischemia
  • Intraluminal suture
  • Nylon
  • Silicon

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Comparison of silicon-coated nylon suture to plain nylon suture in the rat middle cerebral artery occlusion model. / Shimamura, Norihito; Matchett, Gerald; Tsubokawa, Tamiji; Ohkuma, Hiroki; Zhang, John.

In: Journal of Neuroscience Methods, Vol. 156, No. 1-2, 30.09.2006, p. 161-165.

Research output: Contribution to journalArticle

Shimamura, Norihito ; Matchett, Gerald ; Tsubokawa, Tamiji ; Ohkuma, Hiroki ; Zhang, John. / Comparison of silicon-coated nylon suture to plain nylon suture in the rat middle cerebral artery occlusion model. In: Journal of Neuroscience Methods. 2006 ; Vol. 156, No. 1-2. pp. 161-165.
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